验证数据展示
经过测试的应用
| Positive WB detected in | Ramos cells, Raji cells, Daudi cells, HeLa cells, THP-1 cells, human testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 1 publications below |
产品信息
66672-1-Ig targets PLZF in WB, IF, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, rat |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag25180 Product name: Recombinant human PLZF protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 101-400 aa of BC026902 Sequence: DLLYAAEILEIEYLEEQCLKMLETIQASDDNDTEATMADGGAEEEEDRKARYLKNIFISKHSSEESGYASVAGQSLPGPMVDQSPSVSTSFGLSAMSPTKAAVDSLMTIGQSLLQGTLQPPAGPEEPTLAGGGRHPGVAEVKTEMMQVDEVPSQDSPGAAESSISGGMGDKVEERGKEGPGTPTRSSVITSARELHYGREESAEQVPPPAEAGQAPTGRPEHPAPPPEKHLGIYSVLPNHKADAVLSMPSSVTSGLHVQPALAVSMDFSTYGGLLPQGFIQRELFSKLGELAVGMKSESR 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger and BTB domain containing 16 |
| 别名 | ZBTB16, 2E6F12, Zinc finger and BTB domain-containing protein 16, Zinc finger protein 145, Zinc finger protein PLZF |
| 计算分子量 | 673 aa, 74 kDa |
| 观测分子量 | 75 kDa |
| GenBank蛋白编号 | BC026902 |
| 基因名称 | PLZF |
| Gene ID (NCBI) | 7704 |
| RRID | AB_2882026 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q05516 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLZF, also named as Zinc finger protein 145, is a 673 amino acid protein, which belongs to the krueppel C2H2-type zinc-finger protein family. PLZF is expressed in bone marrow, early myeloid cell lines and peripheral blood mononuclear cells. PLZF may play a role in myeloid maturation and in the development and/or maintenance of other differentiated tissues.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLZF antibody 66672-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability | ||
J Hazard Mater Prenatal and postnatal exposure to polystyrene microplastics induces testis developmental disorder and affects male fertility in mice | ||
Med Microbiol Immunol Effects of acetate-producing Blautia wexlerae on oxidative stress and NLRP3 inflammasome in obesity-associated male infertility | ||
Biomed J Autophagy-induced osteoblast-derived exosomes maintain bone formation and prevent osteoporosis by remodeling gut microbiota-metabolism |
