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验证数据展示

  • WB analysis using 29440-1-AP

    WB analysis using 29440-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29440-1-AP (PLXNB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 29440-1-AP

    IHC staining of mouse brain using 29440-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29440-1-AP (PLXNB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLXNB1 antibody included

PLXNB1 Polyclonal antibody

PLXNB1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29440-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

KIAA0407, plexin B1, PLXN5, PLXNB1, Semaphorin receptor SEP, SEP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PLXNB1 Ready-To-Use IHC Kit. PLXNB1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29440-1-AP targets PLXNB1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 PLXNB1 fusion protein Ag31224 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 plexin B1
别名 KIAA0407, plexin B1, PLXN5, PLXNB1, Semaphorin receptor SEP, SEP
计算分子量 2135 aa, 232 kDa
观测分子量 200-250 kDa
GenBank蛋白编号BC146793
基因名称 PLXNB1
Gene ID (NCBI) 5364
RRIDAB_2918306
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43157
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB1 is one of the three plexin-B family members. It is a high-affinity receptor for semaphorin 4D (SEMA4D). Interaction of SEMA4D and PLXNB1 has important physiologic effects in the immune and nervous systems, and is also involved in tumor progression, especially in tumor angiogenesis (PMID: 20858260).

实验方案

Product Specific Protocols
WB protocol for PLXNB1 antibody 29440-1-APDownload protocol
IHC protocol for PLXNB1 antibody 29440-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols