• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PLXDC2 Polyclonal antibody

PLXDC2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 12285-1-AP

Print datasheet

Synonyms

plexin domain containing 2, PLXDC2, TEM7R

验证数据展示

  • IHC staining of human lung cancer using 12285-1-AP

    IHC staining of human lung cancer using 12285-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12285-1-AP (PLXDC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 12285-1-AP

    IHC staining of human lung cancer using 12285-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12285-1-AP (PLXDC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of A431 using 12285-1-AP

    WB analysis of A431 using 12285-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 12285-1-AP (PLXDC2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA431 cells, HeLa cells
Positive IHC detected inhuman lung cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12285-1-AP targets PLXDC2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PLXDC2 fusion protein Ag2940 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name plexin domain containing 2
Synonyms plexin domain containing 2, PLXDC2, TEM7R
Calculated Molecular Weight 480 aa, 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC012885
Gene Symbol PLXDC2
Gene ID (NCBI) 84898
RRIDAB_2166239
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UX71
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLXDC2 (Plexin-domain containing 2), also named tumor endothelial marker 7-related protein (TEM7R), is a single-pass type I membrane protein with some homology to nidogen and to plexins. PLXDC2 is located in tumor endothelium and plays a role in tumor angiogenesis. Three isoforms of PLXDC2 produced by alternative splicing have been described. Recent finding revealed that PLXDC2 is a mitogen for neural progenitors and functions as a novel regulator of the development of different brain regions, suggesting its role in coordinating proliferation and differentiation in the developing nervous system.

实验方案

Product Specific Protocols
WB protocol for PLXDC2 antibody 12285-1-APDownload protocol
IHC protocol for PLXDC2 antibody 12285-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF

Front Cell Dev Biol

Overexpression of PLXDC2 in Stromal Cell-Associated M2 Macrophages Is Related to EMT and the Progression of Gastric Cancer.

Authors - Yiming Guan
View Article
humanWB,IHC

Cancer Res Commun

Adaptive c-Met-PLXDC2 Signaling Axis Mediates Cancer Stem Cell Plasticity to Confer Radioresistance-associated Aggressiveness in Head and Neck Cancer

Authors - Liwei Lang
View Article