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验证数据展示

  • WB analysis of human placenta using 67158-1-Ig

    WB analysis of human placenta using 67158-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67158-1-Ig (PLVAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 67158-1-Ig

    IHC staining of human tonsillitis using 67158-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67158-1-Ig (PLVAP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLVAP antibody included
  • IF Staining of mouse small intestine using 67158-1-Ig

    IF Staining of mouse small intestine using 67158-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using PLVAP antibody (67158-1-Ig, Clone: 1D4A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

PLVAP Monoclonal antibody

PLVAP Monoclonal Antibody for WB, IHC, IF-Fro, ELISA
Cat No. 67158-1-Ig

产品说明书

CloneNo. 1D4A6

宿主/亚型

Mouse / IgG2a

种属反应性

human, pig

应用

WB, IHC, IF-Fro, ELISA

别名

1D4A6, FELS, Fenestrated endothelial-linked structure protein, gp68, Plasmalemma vesicle-associated protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue, pig spleen tissue, pig kidney tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-Fro detected inmouse small intestine tissue
Planning an IHC experiment? We recommend our IHCeasy PLVAP Ready-To-Use IHC Kit. PLVAP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-FROIF-FRO : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67158-1-Ig targets PLVAP in WB, IHC, IF-Fro, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF-Fro, ELISA Application Description
经测试反应性 human, pig
免疫原 PLVAP fusion protein Ag6359 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 plasmalemma vesicle associated protein
别名 1D4A6, FELS, Fenestrated endothelial-linked structure protein, gp68, Plasmalemma vesicle-associated protein
计算分子量 51 kDa
观测分子量 60 kDa
GenBank蛋白编号BC056414
基因名称 PLVAP
Gene ID (NCBI) 83483
RRIDAB_2882455
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BX97
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Plasmalemma vesicle-associated protein (PLVAP) is an endothelial-associated protein that is involved in endothelial diaphragm formation and maintenance of basal vascular permeability. At the same time, PLVAP has roles in immunity by facilitating leukocyte diapedesis at inflammatory sites and controlling peripheral lymph node morphogenesis and the entry into soluble ags into lymph node conduits .(PMID: 36884119, PMID: 37810232)

实验方案

Product Specific Protocols
WB protocol for PLVAP antibody 67158-1-IgDownload protocol
IHC protocol for PLVAP antibody 67158-1-IgDownload protocol
IF protocol for PLVAP antibody 67158-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols