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验证数据展示

  • WB analysis of human placenta using 67158-1-Ig

    WB analysis of human placenta using 67158-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67158-1-Ig (PLVAP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 67158-1-Ig

    IHC staining of human tonsillitis using 67158-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67158-1-Ig (PLVAP antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLVAP antibody included
  • IF Staining of mouse small intestine using 67158-1-Ig

    IF Staining of mouse small intestine using 67158-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse small intestine tissue using PLVAP antibody (67158-1-Ig, Clone: 1D4A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

PLVAP Monoclonal antibody

PLVAP Monoclonal Antibody for WB, IHC, IF-Fro, ELISA
Cat No. 67158-1-Ig

产品说明书

CloneNo. 1D4A6

宿主/亚型

Mouse / IgG2a

种属反应性

human, pig

应用

WB, IHC, IF-Fro, ELISA

别名

1D4A6, FELS, Fenestrated endothelial-linked structure protein, gp68, Plasmalemma vesicle-associated protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue, pig spleen tissue, pig kidney tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-Fro detected inmouse small intestine tissue
Planning an IHC experiment? We recommend our IHCeasy PLVAP Ready-To-Use IHC Kit. PLVAP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-FROIF-FRO : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67158-1-Ig targets PLVAP in WB, IHC, IF-Fro, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF-Fro, ELISA Application Description
经测试反应性 human, pig
免疫原 PLVAP fusion protein Ag6359 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 plasmalemma vesicle associated protein
别名 1D4A6, FELS, Fenestrated endothelial-linked structure protein, gp68, Plasmalemma vesicle-associated protein
计算分子量 51 kDa
观测分子量 60 kDa
GenBank蛋白编号BC056414
基因名称 PLVAP
Gene ID (NCBI) 83483
RRIDAB_2882455
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BX97
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PLVAP antibody 67158-1-IgDownload protocol
IHC protocol for PLVAP antibody 67158-1-IgDownload protocol
IF protocol for PLVAP antibody 67158-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols