验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, L02 cells, mouse liver tissue, rat liver tissue |
Planning an IHC experiment? We recommend our IHCeasy PLVAP Ready-To-Use IHC Kit. PLVAP primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:9000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
| IF | See 1 publications below |
产品信息
14452-1-AP targets PLVAP in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 | PLVAP fusion protein Ag5963 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | plasmalemma vesicle associated protein |
| 别名 | FELS, Fenestrated endothelial-linked structure protein, gp68, Plasmalemma vesicle-associated protein, PV 1 |
| 计算分子量 | 51 kDa |
| 观测分子量 | 50-60 kDa |
| GenBank蛋白编号 | BC056414 |
| 基因名称 | PLVAP |
| Gene ID (NCBI) | 83483 |
| RRID | AB_2166100 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BX97 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Plasmalemma vesicle-associated protein (PLVAP) is an endothelial-associated protein that is involved in endothelial diaphragm formation and maintenance of basal vascular permeability. At the same time, PLVAP has roles in immunity by facilitating leukocyte diapedesis at inflammatory sites and controlling peripheral lymph node morphogenesis and the entry into soluble ags into lymph node conduits .(PMID: 36884119, PMID: 37810232)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLVAP antibody 14452-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuron The Wnt Inhibitor Apcdd1 Coordinates Vascular Remodeling and Barrier Maturation of Retinal Blood Vessels. | ||
J Virol Porcine Epidemic Diarrhea Virus Infection Disrupts the Nasal Endothelial Barrier To Favor Viral Dissemination. | ||
Front Pharmacol Stimulatory G-Protein α Subunit Modulates Endothelial Cell Permeability Through Regulation of Plasmalemma Vesicle-Associated Protein. | ||
Biochem Biophys Res Commun Retinal VEGFA maintains the ultrastructure and function of choriocapillaris by preserving the endothelial PLVAP. | ||
bioRxiv Glutamatergic neuronal activity regulates angiogenesis and blood-retinal barrier maturation via Norrin/β-catenin signaling | ||
Neuron Glutamatergic neuronal activity regulates angiogenesis and blood-retinal barrier maturation via Norrin/β-catenin signaling |