验证数据展示
经过测试的应用
Positive WB detected in | mouse lung tissue |
Positive IP detected in | mouse lung tissue |
Positive IHC detected in | mouse lung tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10413-1-AP targets PLUNC in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IP, IHC, ELISA Application Description |
文献引用应用 | WB, IF, IHC, ChIP |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse |
免疫原 | PLUNC fusion protein Ag0629 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | palate, lung and nasal epithelium associated |
别名 | BPIFA1, LPLUNC3, Lung specific protein X, LUNX, NASG, PLUNC, Protein Plunc, SPLUNC1, SPURT, Von Ebner protein Hl |
计算分子量 | 27 kDa |
观测分子量 | 30 kDa |
GenBank蛋白编号 | BC012549 |
基因名称 | PLUNC |
Gene ID (NCBI) | 51297 |
RRID | AB_2166093 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9NP55 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLUNC, also named as LUNX, NASG and SPURT, belongs to the BPI/LBP/Plunc superfamily and Plunc family. PLUNC may be involved in the airway inflammatory response after exposure to irritants. It is associated with tumor progression. PLUNC plays a role in innate immune responses of the upper airways.
实验方案
Product Specific Protocols | |
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WB protocol for PLUNC antibody 10413-1-AP | Download protocol |
IHC protocol for PLUNC antibody 10413-1-AP | Download protocol |
IP protocol for PLUNC antibody 10413-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Rep Single-cell transcriptomes reveal heterogeneity of chlorine-induced mice acute lung injury and the inhibitory effect of pentoxifylline on ferroptosis | ||
Cell Death Dis USP7 inhibits the progression of nasopharyngeal carcinoma via promoting SPLUNC1-mediated M1 macrophage polarization through TRIM24 | ||
Med Oncol SPLUNC1 regulates LPS-induced progression of nasopharyngeal carcinoma and proliferation of myeloid-derived suppressor cells |