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验证数据展示

  • WB analysis using 60058-1-Ig (same clone as 60058-1-PBS)

    WB analysis using 60058-1-Ig (same clone as 60058-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60058-1-Ig (PLOD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 60058-1-Ig (same clone as 60058-1-PBS)

    WB analysis of human placenta using 60058-1-Ig (same clone as 60058-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 60058-1-Ig (PLOD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 60058-1-Ig (same clone as 60058-1-PBS)

    IHC staining of human pancreas cancer using 60058-1-Ig (same clone as 60058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 60058-1-Ig(PLOD3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 60058-1-Ig (same clone as 60058-1-PBS)

    IHC staining of human pancreas cancer using 60058-1-Ig (same clone as 60058-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 60058-1-Ig(PLOD3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 60058-1-Ig (same clone as 60058-1-PBS)

    WB analysis of HepG2 using 60058-1-Ig (same clone as 60058-1-PBS)

    WB result of PLOD3 antibody (60058-1-Ig, 1:2000) with si-Control and si-PLOD3 transfected HepG2 cells. This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 60058-1-Ig (same clone as 60058-1-PBS)

    IP experiment of HeLa using 60058-1-Ig (same clone as 60058-1-PBS)

    IP result of anti-PLOD3 (IP:60058-1-Ig, 5ug; Detection:60058-1-Ig 1:500) with HeLa cells lysate 1400ug. This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

  • WB analysis using 60058-1-Ig (same clone as 60058-1-PBS)

    WB analysis using 60058-1-Ig (same clone as 60058-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60058-1-Ig (PLOD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60058-1-Ig (same clone as 60058-1-PBS)

    IF Staining of HeLa using 60058-1-Ig (same clone as 60058-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLOD3 antibody (60058-1-Ig, Clone: 2G12B8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60058-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 60058-1-Ig

    Affinity and Kinetic Characterization of 60058-1-Ig

    Biolayer interferometry (BLl) kinetic assays of 60058-1-Ig against Human PLOD3 were performed. The Affinity Constant is below 1 pM.

  • Featured Product
  • KD/KO验证

PLOD3 Monoclonal antibody, PBS Only

PLOD3 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 60058-1-PBS

产品说明书

CloneNo. 2G12B8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

2G12B8, Galactosylhydroxylysine-glucosyltransferase, LH3, Lysyl hydroxylase 3, Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

60058-1-PBS targets PLOD3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1480

Product name: Recombinant human PLOD3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 339-738 aa of BC011674

Sequence: HEPHIADSWPQLQDHFSAVKLVGPEEALSPGEARDMAMDLCRQDPECEFYFSLDADAVLTNLQTLRILIEENRKVIAPMLSRHGKLWSNFWGALSPDEYYARSEDYVELVQRKRVGVWNVPYISQAYVIRGDTLRMELPQRDVFSGSDTDPDMAFCKSFRDKGIFLHLSNQHEFGRLLATSRYDTEHLHPDLWQIFDNPVDWKEQYIHENYSRALEGEGIVEQPCPDVYWFPLLSEQMCDELVAEMEHYGQWSGGRHEDSRLAGGYENVPTVDIHMKQVGYEDQWLQLLRTYVGPMTESLFPGYHTKARAVMNFVVRYRPDEQPSLRPHHDSSTFTLNVALNHKGLDYEGGGCRFLRYDCVISSPRKGWALLHPGRLTHYHEGLPTTWGTRYIMVSFVDP

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3
别名 2G12B8, Galactosylhydroxylysine-glucosyltransferase, LH3, Lysyl hydroxylase 3, Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3
计算分子量 738 aa, 85 kDa
观测分子量 80-85 kDa
GenBank蛋白编号BC011674
基因名称 PLOD3
Gene ID (NCBI) 8985
RRIDAB_2165779
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO60568
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLOD3, also named as LH3, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. The major function of PLOD3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen.

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