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  • Featured Product
  • KD/KO Validated

PLOD2-Specific Polyclonal antibody

PLOD2-Specific Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

FC, IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 21214-1-AP

Print datasheet

Synonyms

LH2, Lysyl hydroxylase 2, PLOD2-Specific, TLH

验证数据展示

  • WB analysis of A431 using 21214-1-AP

    WB analysis of A431 using 21214-1-AP

    WB result of PLOD2-Specific antibody (21214-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLOD2-Specific transfected A431 cells.

  • WB analysis using 21214-1-AP

    WB analysis using 21214-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 21214-1-AP

    WB analysis using 21214-1-AP

    DU 145 cells lysates were subjected to SDS PAGE followed by western blot with 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 21214-1-AP

    IF Staining of HeLa using 21214-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PLOD2-Specific antibody (21214-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of mouse testis using 21214-1-AP

    IP experiment of mouse testis using 21214-1-AP

    IP result of anti-PLOD2-Specific (IP:21214-1-AP, 4ug; Detection:21214-1-AP 1:500) with mouse testis tissue lysate 5000ug.

  • IHC staining of human liver cancer using 21214-1-AP

    IHC staining of human liver cancer using 21214-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLOD2 antibody included
  • IHC staining of human liver cancer using 21214-1-AP

    IHC staining of human liver cancer using 21214-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 21214-1-AP (PLOD2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLOD2 antibody included
  • FC experiment of HeLa using 21214-1-AP

    FC experiment of HeLa using 21214-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PLOD2-Specific (21214-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inCaco-2 cells, A431 cells, DU 145 cells, HT-1080 cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman liver cancer tissue, human pancreas tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PLOD2 Ready-To-Use IHC Kit. PLOD2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21214-1-AP is PLOD2-Specific Fusion Protein expressed in E. coli.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rabbit, zebrafish
Immunogen PLOD2-Specific fusion protein Ag15614 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2
Synonyms LH2, Lysyl hydroxylase 2, PLOD2-Specific, TLH
Calculated Molecular Weight 758 aa, 85 kDa
Observed Molecular Weight 87 kDa
GenBank Accession NumberBC037169
Gene Symbol PLOD2
Gene ID (NCBI) 5352
RRIDAB_10733347
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00469
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy. (PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.This antibody is specific to PLOD2.

实验方案

Product Specific Protocols
WB protocol for PLOD2-Specific antibody 21214-1-APDownload protocol
IHC protocol for PLOD2-Specific antibody 21214-1-APDownload protocol
IF protocol for PLOD2-Specific antibody 21214-1-APDownload protocol
IP protocol for PLOD2-Specific antibody 21214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IHC

J Clin Invest

Lysyl hydroxylase 2 induces a collagen cross-link switch in tumor stroma.

Authors - Yulong Chen
View Article
human, mouseWB

Cancer Discov

Hypoxia-Dependent Modification of Collagen Networks Promotes Sarcoma Metastasis.

Authors - Eisinger-Mathason T S Karin TS
View Article
mouseWB

Arthritis Rheumatol

Therapeutic effect of cyclin-dependent kinase 4/6 inhibitor on dermal fibrosis in murine models of systemic sclerosis.

Authors - Akio Yamamoto
View Article
humanWB

Proc Natl Acad Sci U S A

Intratumoral oxygen gradients mediate sarcoma cell invasion.

Authors - Daniel M Lewis
View Article
mouseIHC

Arthritis Rheum

Gene expression analysis of osteoarthritis synovium reveals elevation of transforming growth factor-beta responsive genes in osteoarthritis-related fibrosis.

Authors - Remst D F G DF
View Article
humanIF,WB,IHC

Cancer Lett

Hypoxic stellate cells of pancreatic cancer stroma regulate extracellular matrix fiber organization and cancer cell motility.

Authors - Masafumi Sada
  • KD Validated
View Article