验证数据展示
经过测试的应用
| Positive WB detected in | DU 145 cells, A431 cells, Caco-2 cells, THLE-2 cells, HEK-293 cells, HeLa cells, HepG2 cells, L02 cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human liver cancer tissue, human pancreas tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21214-1-AP targets PLOD2 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rabbit, zebrafish |
| 免疫原 |
CatNo: Ag15614 Product name: Recombinant human PLOD2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 459-540 aa of BC037169 Sequence: PYMANVYLIKGKTLRSEMNERNYFVRDKLDPDMALCRNAREMTLQREKDSPTPETFQMLSPPKGVFMYISNRHEFGRLLSTA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | procollagen-lysine, 2-oxoglutarate 5-dioxygenase 2 |
| 别名 | PLOD2-Specific, EC:1.14.11.4, Lysyl hydroxylase 2, PLOD2/LH2, Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 |
| 计算分子量 | 758 aa, 85 kDa |
| 观测分子量 | 87 kDa |
| GenBank蛋白编号 | BC037169 |
| 基因名称 | PLOD2 |
| Gene ID (NCBI) | 5352 |
| RRID | AB_10733347 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00469 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLOD2,also named as LH2, forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. It is a potential novel prognostic factor for HCC patients following surgery. Among the PLOD genes, PLOD2 contributes to cancer prognosis and angiogenesis. Several authors have reported that PLOD2 expression might provide prognostic information about malignant tumours such as glioblastoma. PLOD2 expression is a useful biomarker for the effects of antiangiogenic treatment for malignancy. (PMID:22098155). It has 2 isoforms produced by alternative splicing and seven glycosylation sites.This antibody is specific to PLOD2.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for PLOD2 antibody 21214-1-AP | Download protocol |
| IF protocol for PLOD2 antibody 21214-1-AP | Download protocol |
| IHC protocol for PLOD2 antibody 21214-1-AP | Download protocol |
| IP protocol for PLOD2 antibody 21214-1-AP | Download protocol |
| WB protocol for PLOD2 antibody 21214-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Discov Hypoxia-Dependent Modification of Collagen Networks Promotes Sarcoma Metastasis. | ||
Cancer Res A feedback loop between hypoxia and matrix stress relaxation increases oxygen-axis migration and metastasis in sarcoma.
| ||
Cancer Res Sarcoma Cells Secrete Hypoxia-Modified Collagen VI to Weaken the Lung Endothelial Barrier and Promote Metastasis | ||
J Adv Res Targeting PLOD2 induces epithelioid differentiation and improves therapeutic response in sarcomatoid renal cell carcinoma.
| ||
Cell Rep Med Glutathione dynamics is a potential predictive and therapeutic trait for neoadjuvant chemotherapy response in bladder cancer |
