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PLOD1 Polyclonal antibody

PLOD1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29480-1-AP

Print datasheet

Synonyms

FLJ42041, LH, LH1, LLH, Lysyl hydroxylase 1, PLOD, PLOD1

验证数据展示

  • IHC staining of human skin cancer using 29480-1-AP

    IHC staining of human skin cancer using 29480-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 29480-1-AP (PLOD1 antibody) at dilution of 1:200 (under 40x lens).

  • WB analysis using 29480-1-AP

    WB analysis using 29480-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29480-1-AP (PLOD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of K-562 using 29480-1-AP

    IF Staining of K-562 using 29480-1-AP

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using PLOD1 antibody (29480-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA375 cells, K-562 cells, HeLa cells, U-251 cells, U2OS cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29480-1-AP targets PLOD1 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human
Immunogen PLOD1 fusion protein Ag31626 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name procollagen-lysine 1, 2-oxoglutarate 5-dioxygenase 1
Synonyms FLJ42041, LH, LH1, LLH, Lysyl hydroxylase 1, PLOD, PLOD1
Calculated Molecular Weight 727 aa, 84 kDa
Observed Molecular Weight84-88 kDa
GenBank Accession NumberBC016657
Gene Symbol PLOD1
Gene ID (NCBI) 5351
RRIDAB_2918315
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02809
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLOD1, as known as procollagen‐lysine, 2‐oxoglutarate 5‐dioxygenase 1, is a member of the PLOD family(Uniprot, GeneID:5351). PLOD1 participates in catalyzing hydroxylysine residue, which is critical for the formation of covalent cross-link (PMID:30003082). PLOD1 plays an important role in the development and progression of tumors. Aberrantly expressed PLOD1 promotes cancer aggressiveness in glioma and bladder cancer, providing a potential prognostic biomarker and therapeutic target (PMID:31199049, PMID:34040895). PLOD1 has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for PLOD1 antibody 29480-1-APDownload protocol
IHC protocol for PLOD1 antibody 29480-1-APDownload protocol
IF protocol for PLOD1 antibody 29480-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols