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PLK4 Polyclonal antibody

PLK4 Polyclonal Antibody for IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

IF/ICC, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 28750-1-AP

Print datasheet

Synonyms

PLK 4, PLK4, Polo like kinase 4, SAK, STK18

验证数据展示

  • IHC staining of human colon cancer using 28750-1-AP

    IHC staining of human colon cancer using 28750-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28750-1-AP (PLK4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PLK4 antibody included
  • IF Staining of hTERT-RPE1 using 28750-1-AP

    IF Staining of hTERT-RPE1 using 28750-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using PLK4 antibody (28750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red).

经过测试的应用

Positive IHC detected inhuman colon cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells
Planning an IHC experiment? We recommend our IHCeasy PLK4 Ready-To-Use IHC Kit. PLK4 primary antibody included.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28750-1-AP targets PLK4 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications IF/ICC, IHC, ELISA
Cited ApplicationsWB
Tested Reactivity Human, Mouse
Cited Reactivityhuman
Immunogen PLK4 fusion protein Ag30482 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polo-like kinase 4 (Drosophila)
Synonyms PLK 4, PLK4, Polo like kinase 4, SAK, STK18
Calculated Molecular Weight 970 aa, 109 kDa
GenBank Accession NumberBC036023
Gene Symbol PLK4
Gene ID (NCBI) 10733
RRIDAB_2881206
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00444
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLK4 (Polo-like kinase 4) is also named SAK, STK18 and belongs to the Ser/Thr protein kinase family. It plays a central role in centriole duplication and is a kinase implicated in tumor development, which is an upstream regulator of canonical biogenesis necessary for centriole formation. It has 3 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IHC protocol for PLK4 antibody 28750-1-APDownload protocol
IF protocol for PLK4 antibody 28750-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biochem Biophys Res Commun

Advanced glycation end products initiate the mutual promoting cycle between centrosome amplification and the release of inflammatory cytokines in human vascular endothelial cells

Authors - Jun Zhang
  • KD Validated
View Article