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验证数据展示

  • WB analysis using 30486-1-AP (same clone as 30486-1-PBS)

    WB analysis using 30486-1-AP (same clone as 30486-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30486-1-AP (PLK3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30486-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 30486-1-AP (same clone as 30486-1-PBS)

    IP experiment of HeLa using 30486-1-AP (same clone as 30486-1-PBS)

    IP result of anti-PLK3 (IP:30486-1-AP, 4ug; Detection:30486-1-AP 1:500) with HeLa cells lysate 1750 ug. This data was developed using the same antibody clone with 30486-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 30486-1-AP (same clone as 30486-1-PBS)

    IHC staining of human colon using 30486-1-AP (same clone as 30486-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 30486-1-AP (PLK3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30486-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PLK3 antibody included

PLK3 Polyclonal antibody, PBS Only

PLK3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 30486-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

PLK 3, FNK, FGF-inducible kinase, EC:2.7.11.21, Cytokine-inducible serine/threonine-protein kinase

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PLK3 Ready-To-Use IHC Kit. PLK3 primary antibody included.

产品信息

30486-1-PBS targets PLK3 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33186

Product name: Recombinant human PLK3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 550-646 aa of BC013899

Sequence: PSVEEVEVPAPPLLLQWVKTDQALLMLFSDGTVQVNFYGDHTKLILSGWEPLLVTFVARNRSACTYLASHLRQLGCSPDLRQRLRYALRLLRDRSPA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polo-like kinase 3 (Drosophila)
别名 PLK 3, FNK, FGF-inducible kinase, EC:2.7.11.21, Cytokine-inducible serine/threonine-protein kinase
计算分子量 72 kDa
观测分子量 72 kDa
GenBank蛋白编号BC013899
基因名称 PLK3
Gene ID (NCBI) 1263
RRIDAB_3669726
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H4B4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLK3(Polo-like kinase 3) is also named as CNK, FNK, PRK and belongs to the protein kinase superfamily. It is involved in the regulation of DNA damage checkpoint as well as in M-phase function. Plk3 physically interacts with p53 and phosphorylates this tumor suppressor protein on serine-20, suggesting that the role of Plk3 in cell cycle progression is mediated, at least in part, through direct regulation of p53(PMID:12548019).

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