验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, K-562 cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 6 publications below |
| IHC | See 1 publications below |
产品信息
24883-1-AP targets PLEKHO1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse, rat |
| 免疫原 |
CatNo: Ag19309 Product name: Recombinant human PLEKHO1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 176-409 aa of BC010149 Sequence: ASTSTSDGMLTLDLIQEEDPSPEEPTSCAESFRVDLDKSVAQLAGSRRRADSDRIQPSADRASSLSRPWEKTDKGATYTPQAPKKLTPTEKGRCASLEEILSQRDAASARTLQLRAEEPPTPALPNPGQLSRIQDLVARKLEETQELLAEVQGLGDGKRKAKDPPRSPPDSESEQLLLETERLLGEASSNWSQAKRVLQEVRELRDLYRQMDLQTPDSHLRQTTPHSQYRKSLM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pleckstrin homology domain containing, family O member 1 |
| 别名 | C Jun binding protein, CK2 interacting protein 1, CKIP 1, CKIP1, CKIP-1, HQ0024c, JBP, OC120, PLEKHO1 |
| 计算分子量 | 409 aa, 46 kDa |
| 观测分子量 | 46 kDa, 90 kDa |
| GenBank蛋白编号 | BC010149 |
| 基因名称 | PLEKHO1 |
| Gene ID (NCBI) | 51177 |
| RRID | AB_2879778 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q53GL0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLEKHO1, also named as CK2 interacting protein 1 or OC120, is a 409 amino acid protein, which contains one PH domain. PLEKHO1 Predominantly localizes to the plasma membrane. PLEKHO1 plays a role in the regulation of the actin cytoskeleton through its interactions with actin capping protein (CP). Catalog#24883-1-AP recognizes 46 kDa and 90 kDa homodimer band.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLEKHO1 antibody 24883-1-AP | Download protocol |
| IHC protocol for PLEKHO1 antibody 24883-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Bioact Mater Genetically engineered M2-like macrophage-derived exosomes for P. gingivalis-suppressed cementum regeneration: From mechanism to therapy | ||
J Dent Res Ckip-1 Mediates P. gingivalis-Suppressed Cementoblast Mineralization.
| ||
Front Physiol Myocardial CKIP-1 Overexpression Protects from Simulated Microgravity-Induced Cardiac Remodeling. | ||
Front Physiol Casein Kinase-2 Interacting Protein-1 Regulates Physiological Cardiac Hypertrophy via Inhibition of Histone Deacetylase 4 Phosphorylation. | ||
Front Pharmacol Exploratory study of sea buckthorn enhancing QiangGuYin efficacy by inhibiting CKIP-1 and Notum activating the Wnt/β-catenin signaling pathway and analysis of active ingredients by molecular docking | ||
J Oral Microbiol CKIP-1 mediates P. gingivalis-suppressed osteogenic/cementogenic differentiation of periodontal ligament cells partially via p38 signaling pathway |