验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HT-29 cells, mouse brain tissue, mouse cerebellum tissue, rat brain tissue, rat cerebellum tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33028-1-AP targets PLEKHN1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag38741 Product name: Recombinant human PLEKHN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 200-350 aa of BC101386 Sequence: SAPPQGSCGDELPWTLQRRLTRLRTASGHEPGGSAVCASRVKLQHLPAQEQWDRLLVLYPTSLAIFSEELDGLCFKGELPLRAVHINLEEKEKQIRSFLIEGPLINTIRVVCASYEDYGHWLLCLRAVTHREGAPPLPGAESFPGSQVMGS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pleckstrin homology domain containing, family N member 1 |
| 别名 | CLPABP |
| 计算分子量 | 663 aa, 72 kDa |
| 观测分子量 | 66-71 kDa |
| GenBank蛋白编号 | BC101386 |
| 基因名称 | PLEKHN1 |
| Gene ID (NCBI) | 84069 |
| RRID | AB_3742825 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q494U1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLEKHN1 (Pleckstrin Homology Domain Containing N1) is a protein-coding gene located on chromosome 1. It plays a crucial role in various cellular processes, including apoptosis and mRNA stability regulation. PLEKHN1 has been identified as a pro-apoptotic protein that enhances Bax-Bak hetero-oligomerization via interaction with Bid in colon cancer cells. (PMID: 29531808)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLEKHN1 antibody 33028-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
