验证数据展示
经过测试的应用
| Positive WB detected in | Caco-2 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21677-1-AP targets PLEKHG6 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag15886 Product name: Recombinant human PLEKHG6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 456-790 aa of BC105961 Sequence: LMLEKLVCQPLRDPNSFLLIHLTEFQCVSSALLVHCPSPTDRAQWLEKTQQAQAALQKLKAEEYVQQKRELLTLYRDQDRESPSTRPSTPSLEGSQSSAEGRTPEFSTIIPHLVVTEDTDEDAPLVPDDTSDSGYGTLIPGTPTGSRSPLSRLRQRALRRDPRLTFSTLELRDIPLRPHPPDPQAPQRRSAPELPEGILKGGSLPQEDPPTWSEEEDGASERGNVVVETLHRARLRGQLPSSPTHADSAGESPWESSGEEEEEGPLFLKAGHTSLRPMRAEDMLREIREELASQRIEGAEEPRDSRPRKLTRAQLQRMRGPHIIQLDTPLSASEV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | pleckstrin homology domain containing, family G (with RhoGef domain) member 6 |
| 别名 | MyoGEF |
| 计算分子量 | 790 aa, 89 kDa |
| 观测分子量 | 89 kDa |
| GenBank蛋白编号 | BC105961 |
| 基因名称 | PLEKHG6 |
| Gene ID (NCBI) | 55200 |
| RRID | AB_3742049 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q3KR16 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLEKHG6 is a previously unknown guanine nucleotide exchange factor (GEF) for RhoG. It localizes to the apical membrane of cells, a process that depends on its interaction with the membrane-cytoskeleton linker protein ezrin. This apical localization is essential for inducing morphological changes. Additionally, PLEKHG6 and ezrin are both necessary for macropinocytosis mediated by membrane ruffles. Finally, ezrin facilitates precise spatial regulation of RhoG activation at the apical surface of epithelial cells by recruiting components of the RhoG signaling pathway into a closely associated complex.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLEKHG6 antibody 21677-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
