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验证数据展示

  • WB analysis using 32169-1-AP

    WB analysis using 32169-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32169-1-AP (PLEKHA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 32169-1-AP

    IF Staining of A431 using 32169-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using PLEKHA2 antibody (32169-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • IF Staining of HeLa using 32169-1-AP

    IF Staining of HeLa using 32169-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PLEKHA2 antibody (32169-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

PLEKHA2 Polyclonal antibody

PLEKHA2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32169-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

TAPP-2, TAPP2, Tandem PH domain-containing protein 2, Pleckstrin homology domain-containing family A member 2, Pleckstrin Homology Domain Containing A2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, JurKat cells, RT-4 cells
Positive IF/ICC detected inHeLa cells, A431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32169-1-AP targets PLEKHA2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag36596

Product name: Recombinant human PLEKHA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 101-205 aa of BC166639

Sequence: MKDWVEALNQASKITVPKGGGLPMTTEVLKSLAAPPALEKKPQVAYKTEIIGGVVVHTPISQNGGDGQEGSEPGSHTILRRSQSYIPTSGCRASTGPPLIKSGYC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 2
别名 TAPP-2, TAPP2, Tandem PH domain-containing protein 2, Pleckstrin homology domain-containing family A member 2, Pleckstrin Homology Domain Containing A2
观测分子量42 kDa
GenBank蛋白编号BC166639
基因名称 PLEKHA2
Gene ID (NCBI) 59339
RRIDAB_3670213
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9HB19
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLEKHA2 (Pleckstrin homology domain containing A2), also known as TAPP2, is a member of the PLEKHA family of proteins.PLEKHA2 is a junctional protein with a Pleckstrin homology domain that is involved in signaling after lymphocyte activation and plays an important role in cytoskeletal reorganization driven by phosphatidylinositol 3-kinase.

实验方案

Product Specific Protocols
IF protocol for PLEKHA2 antibody 32169-1-APDownload protocol
WB protocol for PLEKHA2 antibody 32169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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