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  • Featured Product
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PLEK Monoclonal antibody

PLEK Monoclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D5C2

Cat No : 66431-1-Ig

Print datasheet

Synonyms

P47, Platelet 47 kDa protein, pleckstrin, PLEK

验证数据展示

  • WB analysis of U-937 using 66431-1-Ig

    WB analysis of U-937 using 66431-1-Ig

    WB result of PLEK antibody (66431-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLEK transfected U-937 cells.

  • IF Staining of mouse spleen using 66431-1-Ig

    IF Staining of mouse spleen using 66431-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using PLEK antibody (66431-1-Ig, Clone: 1D5C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse spleen using 66431-1-Ig

    IF Staining of mouse spleen using 66431-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using PLEK antibody (66431-1-Ig, Clone: 1D5C2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • WB analysis of HL-60 using 66431-1-Ig

    WB analysis of HL-60 using 66431-1-Ig

    HL-60 cells were subjected to SDS PAGE followed by western blot with 66431-1-Ig (PLEK Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 66431-1-Ig

    IHC staining of human colon cancer using 66431-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66431-1-Ig (PLEK Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 66431-1-Ig

    IHC staining of human colon cancer using 66431-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66431-1-Ig (PLEK Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHL-60 cells, pig spleen tissue, THP-1 cells, U-937 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse spleen tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66431-1-Ig targets PLEK in WB, IF, IHC, ELISA applications and shows reactivity with human, pig, mouse samples.

Tested Applications IF-P, IHC, WB, ELISA
Cited ApplicationsIF, IHC
Tested Reactivity human, pig, mouse
Cited Reactivityhuman
Immunogen PLEK fusion protein Ag3187 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name pleckstrin
Synonyms P47, Platelet 47 kDa protein, pleckstrin, PLEK
Calculated Molecular Weight 350 aa, 40 kDa
Observed Molecular Weight 40-47 kDa
GenBank Accession NumberBC018549
Gene Symbol PLEK
Gene ID (NCBI) 5341
RRIDAB_2881802
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08567
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLEK (pleckstrin; also known as p47) was originally identified as the major PKC substrate in platelets and later is found to be expressed in all cells of the hemopoietic system. Through two pleckstrin homology (PH) domains at its N and C termini, PLEK may interact with various protein and/or lipid ligands and serve as an intracellular adaptor/targeting protein. Predominantly cytosolic in unstimulated cells, PLEK would undergo transient redistribution to phagosomal membrane in response to stimuli. PLEK has been found to be hyperphosphorylated in diabetic mononuclear phagocytes and promote proinflammatory cytokine secretion in diabetes. (10477609, 17579087)

实验方案

Product Specific Protocols
WB protocol for PLEK antibody 66431-1-IgDownload protocol
IHC protocol for PLEK antibody 66431-1-IgDownload protocol
IF protocol for PLEK antibody 66431-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF

Aging (Albany NY)

Comprehensive analysis identifies crucial genes associated with immune cells mediating progression of carotid atherosclerotic plaque

Authors - Zhen Li
View Article