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验证数据展示

  • WB analysis using 29170-1-AP (same clone as 29170-1-PBS)

    WB analysis using 29170-1-AP (same clone as 29170-1-PBS)

    Various lysates were subjected to Tris-Acetate gel system followed by western blot with 29170-1-AP (PLEC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29170-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 29170-1-AP (same clone as 29170-1-PBS)

    IHC staining of mouse heart using 29170-1-AP (same clone as 29170-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 29170-1-AP (PLEC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29170-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PLEC antibody included
  • IF Staining of A549 using 29170-1-AP (same clone as 29170-1-PBS)

    IF Staining of A549 using 29170-1-AP (same clone as 29170-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using PLEC antibody (29170-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 29170-1-PBS in a different storage buffer formulation.

PLEC Polyclonal antibody, PBS Only

PLEC Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29170-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PLEC1, Plectin, PLEC1b, Plectin 1, PLTN

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PLEC Ready-To-Use IHC Kit. PLEC primary antibody included.

产品信息

29170-1-PBS targets PLEC in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag29418

Product name: Recombinant human PLEC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1475-1768 aa of NM_201380

Sequence: SETLRRMEEEERLAEQQRAEERERLAEVEAALEKQRQLAEAHAQAKAQAEREAKELQQRMQEEVVRREEAAVDAQQQKRSIQEELQQLRQSSEAEIQAKARQAEAAERSRLRIEEEIRVVRLQLEATERQRGGAEGELQALRARAEEAEAQKRQAQEEAERLRRQVQDESQRKRQAEVELASRVKAEAEAAREKQRALQALEELRLQAEEAERRLRQAEVERARQVQVALETAQRSAEAELQSKRASFAEKTAQLERSLQEEHVAVAQLREEAERRAQQQAEAERAREEAEREL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 plectin 1, intermediate filament binding protein 500kDa
别名 PLEC1, Plectin, PLEC1b, Plectin 1, PLTN
计算分子量 532 kDa
观测分子量 510 kDa
GenBank蛋白编号NM_201380
基因名称 Plectin
Gene ID (NCBI) 5339
RRIDAB_3086100
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15149
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Plectin is a large (≥500-kDa) protein that is normally expressed in various tissues including skin, muscle, and brain. It binds to a number of cytoskeletal proteins including microtubules and intermediate filaments and is involved in establishment and dynamic modulation of the cytoskeletal network. In normal cells, it plays a crucial role in cytoskeleton network organization. In keratinocytes, plectin is concentrated at the basal surface, where it links intermediate filaments to the cytoplasmic domain of transmembrane glycoproteins such as integrin beta-4. (PMID: 24218614, PMID: 28281696, PMID: 23750011)

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