验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, HEK-293 cells, Jurkat cells, ROS1728 cells, RAW 264.7 cells, NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66558-1-Ig targets PLDN in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag18095 Product name: Recombinant human PLDN protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-172 aa of BC004819 Sequence: MSVPGPSSPDGALTRPPYCLEAGEPTPGLSDTSPDEGLIEDLTIEDKAVEQLAEGLLSHYLPDLQRSKQALQELTQNQVVLLDTLEQEISKFKECHSMLDINALFAEAKHYHAKLVNIRKEMLMLHEKTSKLKKRALKLQQKRQKEELEREQQREKEFEREKQLTARPAKRM 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | pallidin homolog (mouse) |
| 别名 | Pallidin, 4F4C11, BLOC-1 subunit 6, BLOC1S6, HPS9 |
| 计算分子量 | 23~24 kDa |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | BC004819 |
| 基因名称 | PLDN |
| Gene ID (NCBI) | 26258 |
| RRID | AB_2881919 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9UL45 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Pallidin, also named as PLDN and PA, is involved in the development of lysosome-related organelles, such as melanosomes and platelet-dense granules. It may play a role in intracellular vesicle trafficking, particularly in the vesicle-docking and fusion process. Pallidin interacts with syntaxin-12 which is a t-SNARE protein that mediates vesicle docking and fusion.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLDN antibody 66558-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |