验证数据展示
经过测试的应用
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A549 cells, U2OS cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20161-1-AP targets PLCXD1 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | IHC, IF/ICC, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | PLCXD1 fusion protein Ag14086 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | phosphatidylinositol-specific phospholipase C, X domain containing 1 |
别名 | phosphatidylinositol phospholipase C, X domain containing 1, PI-PLC X domain-containing protein 1 |
计算分子量 | 323 aa, 37 kDa |
GenBank蛋白编号 | BC005028 |
基因名称 | PLCXD1 |
Gene ID (NCBI) | 55344 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9NUJ7 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Phosphatidylinositol-specific phospholipase C (PI-PLC) enzymes comprise a small family of receptor-regulated phosphodiesterases that control many cellular processes by the regulation of cytosolic calcium and/or the activity of several protein kinases. At least three tissue-specific PLCXD isoforms exist in humans, comprising hPLCXD-1, hPLCXD-2 and hPLCXD-3 (PMID: 22732399). Mutations in the PLCXD1 gene are associated with diseases such as Leri-Weill Dyschondrosteosis.
实验方案
Product Specific Protocols | |
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IHC protocol for PLCXD1 antibody 20161-1-AP | Download protocol |
IF protocol for PLCXD1 antibody 20161-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |