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PLCL2 Monoclonal antibody

PLCL2 Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat, Mouse, Pig

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1C8A4

Cat No : 67471-1-Ig

Print datasheet

Synonyms

FLJ13484, KIAA1092, Phospholipase C epsilon 2, Phospholipase C L2, phospholipase C like 2, PLC epsilon 2, PLC L(2), PLC L2, PLCE2, PLCL2

验证数据展示

  • WB analysis using 67471-1-Ig

    WB analysis using 67471-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67471-1-Ig (PLCL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67471-1-Ig

    IF Staining of HeLa using 67471-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 67471-1-Ig (PLCL2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inpig brain tissue, mouse brain tissue, rat brain tissue
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67471-1-Ig targets PLCL2 in WB, IF, ELISA applications and shows reactivity with Human, Rat, Mouse, Pig samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, Rat, Mouse, Pig
Immunogen PLCL2 fusion protein Ag11461 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phospholipase C-like 2
Synonyms FLJ13484, KIAA1092, Phospholipase C epsilon 2, Phospholipase C L2, phospholipase C like 2, PLC epsilon 2, PLC L(2), PLC L2, PLCE2, PLCL2
Calculated Molecular Weight 126 kDa
Observed Molecular Weight 126 kDa
GenBank Accession NumberBC036392
Gene Symbol PLCL2
Gene ID (NCBI) 23228
RRIDAB_2882700
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UPR0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLCL2, a novel phospholipase C-like protein, is expressed in lymphocytes and platelets. The expression of PLCL2 is associated with the proliferation of mature B cells in the immune system. PLCL2 is a new susceptibility loci for myocardial infarction. It has been shown that PLCL2 plays a key role in the pathogenesis of atherosclerosis and systemic sclerosisit. There are three isoforms of PLCL2 protein and 67471-1-Ig antibody detects the 126 kDa band in SDS-PAGE. (PMID: 24916648, 25880423)

实验方案

Product Specific Protocols
WB protocol for PLCL2 antibody 67471-1-IgDownload protocol
IF protocol for PLCL2 antibody 67471-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols