验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, SH-SY5Y cells |
Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | Hela cells, HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
19143-1-AP targets PLCH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | PLCH1 fusion protein Ag13607 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | phospholipase C, eta 1 |
别名 | Phospholipase C-eta-1, phospholipase C, eta 1, Phospholipase C like protein 3, Phosphoinositide phospholipase C-eta-1, EC:3.1.4.11 |
计算分子量 | 1655 aa, 185 kDa |
观测分子量 | 185 kDa |
GenBank蛋白编号 | BC113950 |
基因名称 | PLCH1 |
Gene ID (NCBI) | 23007 |
RRID | AB_10643386 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q4KWH8 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for PLCH1 antibody 19143-1-AP | Download protocol |
IHC protocol for PLCH1 antibody 19143-1-AP | Download protocol |
IF protocol for PLCH1 antibody 19143-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |