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验证数据展示

  • WB analysis using 19143-1-AP

    WB analysis using 19143-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19143-1-AP (PLCH1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 19143-1-AP

    IHC staining of human brain using 19143-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19143-1-AP (PLCH1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 19143-1-AP

    IHC staining of human brain using 19143-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19143-1-AP (PLCH1 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 19143-1-AP

    IF Staining of HeLa using 19143-1-AP

    Immunofluorescent analysis of Hela cells, using PLCH1 antibody 19143-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

PLCH1 Polyclonal antibody

PLCH1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19143-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Phospholipase C-eta-1, phospholipase C, eta 1, Phospholipase C like protein 3, Phosphoinositide phospholipase C-eta-1, EC:3.1.4.11

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, SH-SY5Y cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19143-1-AP targets PLCH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 PLCH1 fusion protein Ag13607 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase C, eta 1
别名 Phospholipase C-eta-1, phospholipase C, eta 1, Phospholipase C like protein 3, Phosphoinositide phospholipase C-eta-1, EC:3.1.4.11
计算分子量 1655 aa, 185 kDa
观测分子量 185 kDa
GenBank蛋白编号BC113950
基因名称 PLCH1
Gene ID (NCBI) 23007
RRIDAB_10643386
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ4KWH8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PLCH1 antibody 19143-1-APDownload protocol
IHC protocol for PLCH1 antibody 19143-1-APDownload protocol
IF protocol for PLCH1 antibody 19143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Immunol

Identification of nine signature proteins involved in periodontitis by integrated analysis of TMT proteomics and transcriptomics

Authors - Wei Liu
View Article