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验证数据展示

  • WB analysis of HeLa using 30133-1-AP

    WB analysis of HeLa using 30133-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 30133-1-AP (PLCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 30133-1-AP

    WB analysis of HeLa using 30133-1-AP

    WB result of PLCD4 antibody (30133-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PLCD4 transfected HeLa cells.

  • IHC staining of mouse testis using 30133-1-AP

    IHC staining of mouse testis using 30133-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30133-1-AP (PLCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 30133-1-AP

    IHC staining of human pancreas cancer using 30133-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 30133-1-AP (PLCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

PLCD4 Polyclonal antibody

PLCD4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30133-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, rat, mouse

应用

WB, IHC, ELISA

别名

hPLCD4, Phospholipase C delta 4, phospholipase C, delta 4, PLC delta 4, PLCD4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, rat testis tissue, human testis tissue
Positive IHC detected inmouse testis tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30133-1-AP targets PLCD4 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, rat, mouse
免疫原

CatNo: Ag32651

Product name: Recombinant human PLCD4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-290 aa of BC006355

Sequence: MASLLQDQLTTDQDLLLMQEGMPMRKVRSKSWKKLRYFRLQNDGMTVWHARQARGSAKPSFSISDVETIRNGHDSELLRSLAEELPLEQGFTIVFHGRRSNLDLMANSVEEAQIWMRGLQLLVDLVTSMDHQERLDQWLSDWFQRGDKNQDGKMSFQEVQRLLHLMNVEMDQEYAFSLFQAADTSQSGTLEGEEFVQFYKALTKRAEVQELFESFSADGQKLTLLEFLDFLQEEQKERDCTSELALELIDRYEPSDSGKLRHVLSMDGFLSYLCSKDGDIFNPACLPIYQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase C, delta 4
别名 hPLCD4, Phospholipase C delta 4, phospholipase C, delta 4, PLC delta 4, PLCD4
计算分子量 85-90 kDa
观测分子量90 kDa
GenBank蛋白编号BC006355
基因名称 PLCD4
Gene ID (NCBI) 84812
RRIDAB_2935518
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BRC7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLCD4(1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-4), also known as hPLCD4. It is highly expressed in skeletal muscle and kidney tissues. It may be an important enzyme for intracellular Ca²⁺ mobilization in the zona pellucida-induced acrosome reaction. PLCD4 cooperates with nuclear PKC to regulate liver regeneration. Overexpression of PLCD4 upregulates Erk signaling pathway and proliferation (PMID: 15140260).

实验方案

Product Specific Protocols
WB protocol for PLCD4 antibody 30133-1-APDownload protocol
IHC protocol for PLCD4 antibody 30133-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols