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验证数据展示

  • WB analysis of mouse skeletal muscle using 10589-2-AP

    WB analysis of mouse skeletal muscle using 10589-2-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10589-2-AP (PLCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 10589-2-AP

    IHC staining of human pancreas cancer using 10589-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10589-2-AP (PLCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 10589-2-AP

    IHC staining of human pancreas cancer using 10589-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10589-2-AP (PLCD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PLCD4 Polyclonal antibody

PLCD4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10589-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

hPLCD4, Phospholipase C delta 4, phospholipase C, delta 4, PLC delta 4, PLCD4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10589-2-AP targets PLCD4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0937

Product name: Recombinant human PLCD4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 462-762 aa of BC006355

Sequence: ESQFETEPEPQEQNLQNKDKKKKSKPILCPALSSLVIYLKSVSFRSFTHSKEHYHFYEISSFSETKAKRLIKEAGNEFVQHNTWQLSRVYPSGLRTDSSNYNPQELWNAGCQMVAMNMQTAGLEMDICDGHFRQNGGCGYVLKPDFLRDIQSSFHPEKPISPFKAQTLLIQVISGQQLPKVDKTKEGSIVDPLVKVQIFGVRLDTARQETNYVENNGFNPYWGQTLCFRVLVPELAMLRFVVMDYDWKSRNDFIGQYTLPWTCMQQGYRHIHLLSKDGISLRPASIFVYICIQEGLEGDES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase C, delta 4
别名 hPLCD4, Phospholipase C delta 4, phospholipase C, delta 4, PLC delta 4, PLCD4
计算分子量 85-90 kDa
观测分子量100-110 kDa
GenBank蛋白编号BC006355
基因名称 PLCD4
Gene ID (NCBI) 84812
RRIDAB_2163398
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BRC7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLCD4 is a member of the delta class of phospholipase C enzymes. Phospholipase C enzymes play a critical role in many cellular processes by hydrolyzing phosphatidylinositol 4,5-bisphosphate into two intracellular second messengers, inositol 1,4,5-trisphosphate and diacylglycerol. Expression of this gene may be a marker for cancer.

实验方案

Product Specific Protocols
WB protocol for PLCD4 antibody 10589-2-APDownload protocol
IHC protocol for PLCD4 antibody 10589-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols