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验证数据展示

  • WB analysis of human testis using 67064-1-Ig

    WB analysis of human testis using 67064-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 67064-1-Ig (PLCD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 67064-1-Ig

    IHC staining of human testis using 67064-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 67064-1-Ig (PLCD1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human testis using 67064-1-Ig

    IHC staining of human testis using 67064-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 67064-1-Ig (PLCD1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of K-562 using 67064-1-Ig

    FC experiment of K-562 using 67064-1-Ig

    1x10^6 K-562 cells were intracellularly stained with 0.2 ug PLCD1 Monoclonal antibody (67064-1-Ig, Clone:1D10B11) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

PLCD1 Monoclonal antibody

PLCD1 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 67064-1-Ig

产品说明书

CloneNo. 1D10B11

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, FC (Intra), ELISA

别名

1D10B11, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1, EC:3.1.4.11, Phosphoinositide phospholipase C-delta-1, Phospholipase C delta 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman testis tissue, K-562 cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inK-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67064-1-Ig targets PLCD1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5567

Product name: Recombinant human PLCD1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 412-756 aa of BC050382

Sequence: MLLNRPLDGVTNSLPSPEQLKGKILLKGKKLGGLLPPGGEGGPEATVVSDEDEAAEMEDEAVRSRVQHKPKEDKLRLAQELSDMVIYCKSVHFGGFSSPGTPGQAFYEMASFSENRALRLLQESGNGFVRHNVGHLSRIYPAGWRTDSSNYSPVEMWNGGCQIVALNFQTPGPEMDVYQGRFQDNGACGYVLKPAFLRDPNGTFNPRALAQGPWWARKRLNIRVISGQQLPKVNKNKNSIVDPKVTVEIHGVSRDVASRQTAVITNNGFNPWWDTEFAFEVVVPDLALIRFLVEDYDASSKNDFIGQSTIPLNSLKQGYRHVHLMSKNGDQHPSATLFVKISLQD

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phospholipase C, delta 1
别名 1D10B11, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1, EC:3.1.4.11, Phosphoinositide phospholipase C-delta-1, Phospholipase C delta 1
计算分子量 86 kDa
观测分子量 85 kDa
GenBank蛋白编号BC050382
基因名称 PLCD1
Gene ID (NCBI) 5333
RRIDAB_2882374
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP51178
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PLCD1 antibody 67064-1-IgDownload protocol
IHC protocol for PLCD1 antibody 67064-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols