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验证数据展示

  • WB analysis of HeLa using 51139-1-AP

    WB analysis of HeLa using 51139-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 51139-1-AP (PLCB3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of MDA-MB-231 using 51139-1-AP

    IP experiment of MDA-MB-231 using 51139-1-AP

    IP result of anti-PLCB3 (IP:51139-1-AP, 4ug; Detection:51139-1-AP 1:1000) with MDA-MB-231 cells lysate 1280 ug.

PLCB3 Polyclonal antibody

PLCB3 Polyclonal Antibody for WB, IP, ELISA
Cat No. 51139-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3, EC:3.1.4.11, Phosphoinositide phospholipase C-beta-3, Phospholipase C beta 3, Phospholipase C-beta-3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, MCF-7 cells
Positive IP detected inMDA-MB-231 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51139-1-AP targets PLCB3 in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase C, beta 3 (phosphatidylinositol-specific)
别名 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3, EC:3.1.4.11, Phosphoinositide phospholipase C-beta-3, Phospholipase C beta 3, Phospholipase C-beta-3
计算分子量 1234 aa, 139 kDa
观测分子量 139 kDa
GenBank蛋白编号BC142681
基因名称 PLCB3
Gene ID (NCBI) 5331
RRIDAB_2881250
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ01970
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLCB3 is a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Six subfamilies of PLCs (B, G, D, E, Z and H) constitute part of ubiquitous signaling cascades that translate hormonal signals into intracellular events, leading to alternations in cell function. PLCB isoforms 1-4 are stimulated by G-protein activation (Gαq/11 and/or Gβγ). Independent of its enzymatic activity, PLCB3 inhibits the proliferation of hematopoietic stem cells (HSCs) and myeloid cells.

实验方案

Product Specific Protocols
WB protocol for PLCB3 antibody 51139-1-APDownload protocol
IP protocol for PLCB3 antibody 51139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols