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验证数据展示

  • WB analysis of DU 145 using 26835-1-AP

    WB analysis of DU 145 using 26835-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 26835-1-AP (PLB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate hyperplasia using 26835-1-AP

    IHC staining of human prostate hyperplasia using 26835-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 26835-1-AP (PLB1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human prostate hyperplasia using 26835-1-AP

    IHC staining of human prostate hyperplasia using 26835-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 26835-1-AP (PLB1 Antibody) at dilution of 1:200 (under 40x lens).

PLB1 Polyclonal antibody

PLB1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26835-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Phospholipase A2, Lysophospholipase, hPLB, EC:3.1.1.5, EC:3.1.1.4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inDU 145 cells
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26835-1-AP targets PLB1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag25240

Product name: Recombinant human PLB1 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 22-372 aa of BC150633

Sequence: QIHTSPRKSTLEGQLWPETLKNSPFPCNPNKLGVNMPSKSVHSLKPSDIKFVAAIGNLEIPPDPGTGDLEKQDWTERPQQVCMGVMTVLSDIIRYFSPSVPMPVCHTGKRVIPHDGAEDLWIQAQELVRNMKENLQLDFQFDWKLINVFFSNASQCYLCPSAQQNGLAAGGVDELMGVLDYLQQEVPRAFVNLVDLSEVAEVSRQYHGTWLSPAPEPCNCSEETTRLAKVVMQWSYQEAWNSLLASSRYSEQESFTVVFQPFFYETTPSLHSEDPRLQDSTTLAWHLWNRMMEPAGEKDEPLSVKHGRPMKCPSQESPYLFSYRNSNYLTRLQKPQDKLEVREGAEIRCPD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase B1
别名 Phospholipase A2, Lysophospholipase, hPLB, EC:3.1.1.5, EC:3.1.1.4
观测分子量 45 kDa
GenBank蛋白编号BC150633
基因名称 PLB1
Gene ID (NCBI) 151056
RRIDAB_2880652
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6P1J6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PLB1 antibody 26835-1-APDownload protocol
IHC protocol for PLB1 antibody 26835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols