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PLB1 Polyclonal antibody

PLB1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 26835-1-AP

Print datasheet

Synonyms

hPLB, Phospholipase B, Phospholipase B/lipase, phospholipase B1, PLB, PLB1

验证数据展示

  • WB analysis of DU 145 using 26835-1-AP

    WB analysis of DU 145 using 26835-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 26835-1-AP (PLB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate hyperplasia using 26835-1-AP

    IHC staining of human prostate hyperplasia using 26835-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 26835-1-AP (PLB1 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human prostate hyperplasia using 26835-1-AP

    IHC staining of human prostate hyperplasia using 26835-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 26835-1-AP (PLB1 Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inDU 145 cells
Positive IHC detected inhuman prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26835-1-AP is PLB1 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human
Immunogen PLB1 fusion protein Ag25240 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phospholipase B1
Synonyms hPLB, Phospholipase B, Phospholipase B/lipase, phospholipase B1, PLB, PLB1
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC150633
Gene Symbol PLB1
Gene ID (NCBI) 151056
RRIDAB_2880652
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P1J6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PLB1 antibody 26835-1-APDownload protocol
IHC protocol for PLB1 antibody 26835-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols