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验证数据展示

  • WB analysis of A431 using 18018-1-AP

    WB analysis of A431 using 18018-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 18018-1-AP (PLAG1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 18018-1-AP

    IP experiment of HepG2 using 18018-1-AP

    IP result of anti-PLAG1 (IP:18018-1-AP, 4ug; Detection:18018-1-AP 1:300) with HepG2 cells lysate 2400ug.

  • IF Staining of HepG2 using 18018-1-AP

    IF Staining of HepG2 using 18018-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 18018-1-AP (PLAG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PLAG1 Polyclonal antibody

PLAG1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 18018-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA

别名

Zinc finger protein PLAG1, SGPA, PSA, pleiomorphic adenoma gene 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18018-1-AP targets PLAG1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12566

Product name: Recombinant human PLAG1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 151-500 aa of BC075047

Sequence: TCKVCLQTFESTGVLLEHLKSHAGKSSGGVKEKKHQCEHCDRRFYTRKDVRRHMVVHTGRKDFLCQYCAQRFGRKDHLTRHMKKSHNQELLKVKTEPVDFLDPFTCNVSVPIKDELLPVMSLPSSELLSKPFTNTLQLNLYNTPFQSMQSSGSAHQMITTLPLGMTCPIDMDTVHPSHHLSFKYPFSSTSYAISIPEKEQPLKGEIESYLMELQGGVPSSSQDSQASSSSKLGLDPQIGSLDDGAGDLSLSKSSISISDPLNTPALDFSQLFNFIPLNGPPYNPLSVGSLGMSYSQEEAHSSVSQLPPQTQDLQDPANTIGLGSLHSLSAAFTSSLSTSTTLPRFHQAFQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pleiomorphic adenoma gene 1
别名 Zinc finger protein PLAG1, SGPA, PSA, pleiomorphic adenoma gene 1
计算分子量 500 aa, 56 kDa
观测分子量 56 kDa
GenBank蛋白编号BC075047
基因名称 PLAG1
Gene ID (NCBI) 5324
RRIDAB_2878482
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6DJT9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for PLAG1 antibody 18018-1-APDownload protocol
IP protocol for PLAG1 antibody 18018-1-APDownload protocol
WB protocol for PLAG1 antibody 18018-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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