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验证数据展示

  • WB analysis using 66752-1-Ig (same clone as 66752-1-PBS)

    WB analysis using 66752-1-Ig (same clone as 66752-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66752-1-Ig (PLA2G4D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66752-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66752-1-Ig (same clone as 66752-1-PBS)

    IF Staining of HepG2 using 66752-1-Ig (same clone as 66752-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PLA2G4D antibody (66752-1-Ig, Clone: 2E2A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66752-1-PBS in a different storage buffer formulation.

PLA2G4D Monoclonal antibody, PBS Only

PLA2G4D Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66752-1-PBS

产品说明书

CloneNo. 2E2A10

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IF/ICC, Indirect ELISA

别名

cPLA2 delta, cPLA2delta, Phospholipase A2 group IVD, PLA2G4D

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66752-1-PBS targets PLA2G4D in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag4178

Product name: Recombinant human PLA2G4D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-216 aa of BC034571

Sequence: MGRLMRRIPEPRICFLEAIWSNIFSLNLLDAWYDLTSSGESWKQHIKDKTRSLEKEPLTTSGTSSRLEASWLQPGTALAQAFKGFLTGRPLHQRSPNFLQGLQLHQDYCSHKDFSTWADYQLDSMPSQLTPKEPRLCLVDAAYFINTSSPSMFRPGRRLDLILSFDYSLSAPFEVPWSPQGNPSAQPGQAPEASSRATEPLPHTARVPKGRRGVRP

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phospholipase A2, group IVD (cytosolic)
别名 cPLA2 delta, cPLA2delta, Phospholipase A2 group IVD, PLA2G4D
计算分子量 818 aa, 92 kDa
观测分子量 92 kDa
GenBank蛋白编号BC034571
基因名称 PLA2G4D
Gene ID (NCBI) 283748
RRIDAB_2882098
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ86XP0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLA2G4D(Cytosolic phospholipase A2 delta) belongs to the phospholipase A2 enzyme family,catalyzes the hydrolysis of glycerophospholipids at the sn-2 position and then liberate free fatty acids and lysophospholipids.PLA2G4D expression is specifically upregulated in the upper epidermis of patients with inflammatory skin diseases(PMID:14709560).

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