验证数据展示
经过测试的应用
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
13345-1-AP targets PLA2G4D in IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag4178 Product name: Recombinant human PLA2G4D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-216 aa of BC034571 Sequence: MGRLMRRIPEPRICFLEAIWSNIFSLNLLDAWYDLTSSGESWKQHIKDKTRSLEKEPLTTSGTSSRLEASWLQPGTALAQAFKGFLTGRPLHQRSPNFLQGLQLHQDYCSHKDFSTWADYQLDSMPSQLTPKEPRLCLVDAAYFINTSSPSMFRPGRRLDLILSFDYSLSAPFEVPWSPQGNPSAQPGQAPEASSRATEPLPHTARVPKGRRGVRP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phospholipase A2, group IVD (cytosolic) |
| 别名 | cPLA2 delta, cPLA2delta, cPLA2-delta, EC:3.1.1.4, PLA2G4 |
| 计算分子量 | 818 aa, 92 kDa |
| 观测分子量 | 92 kDa |
| GenBank蛋白编号 | BC034571 |
| 基因名称 | PLA2G4D |
| Gene ID (NCBI) | 283748 |
| RRID | AB_2164607 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86XP0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLA2G4D(Cytosolic phospholipase A2 delta) belongs to the phospholipase A2 enzyme family,catalyzes the hydrolysis of glycerophospholipids at the sn-2 position and then liberate free fatty acids and lysophospholipids.PLA2G4D expression is specifically upregulated in the upper epidermis of patients with inflammatory skin diseases(PMID:14709560).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PLA2G4D antibody 13345-1-AP | Download protocol |
| WB protocol for PLA2G4D antibody 13345-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
