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验证数据展示

  • WB analysis of mouse brain using 13345-1-AP

    WB analysis of mouse brain using 13345-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13345-1-AP (PLA2G4D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 13345-1-AP

    IHC staining of human skin cancer using 13345-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13345-1-AP (PLA2G4D Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin cancer using 13345-1-AP

    IHC staining of human skin cancer using 13345-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13345-1-AP (PLA2G4D Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PLA2G4D Polyclonal antibody

PLA2G4D Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13345-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

Cpla2 delta, cPLA2delta, Phospholipase A2 group IVD, PLA2G4D

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, Transfected HEK-293 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13345-1-AP targets PLA2G4D in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4178

Product name: Recombinant human PLA2G4D protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-216 aa of BC034571

Sequence: MGRLMRRIPEPRICFLEAIWSNIFSLNLLDAWYDLTSSGESWKQHIKDKTRSLEKEPLTTSGTSSRLEASWLQPGTALAQAFKGFLTGRPLHQRSPNFLQGLQLHQDYCSHKDFSTWADYQLDSMPSQLTPKEPRLCLVDAAYFINTSSPSMFRPGRRLDLILSFDYSLSAPFEVPWSPQGNPSAQPGQAPEASSRATEPLPHTARVPKGRRGVRP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase A2, group IVD (cytosolic)
别名 Cpla2 delta, cPLA2delta, Phospholipase A2 group IVD, PLA2G4D
计算分子量 818 aa, 92 kDa
观测分子量 92 kDa
GenBank蛋白编号BC034571
基因名称 PLA2G4D
Gene ID (NCBI) 283748
RRIDAB_2164607
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86XP0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PLA2G4D(Cytosolic phospholipase A2 delta) belongs to the phospholipase A2 enzyme family,catalyzes the hydrolysis of glycerophospholipids at the sn-2 position and then liberate free fatty acids and lysophospholipids.PLA2G4D expression is specifically upregulated in the upper epidermis of patients with inflammatory skin diseases(PMID:14709560).

实验方案

Product Specific Protocols
IHC protocol for PLA2G4D antibody 13345-1-APDownload protocol
WB protocol for PLA2G4D antibody 13345-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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