验证数据展示
经过测试的应用
| Positive WB detected in | mouse pancreas tissue, mouse spleen tissue, rat pancreas tissue, pig pancreas tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse pancreas tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
15843-1-AP targets PLA2G1B in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Ag8603 Product name: Recombinant human PLA2G1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-70 aa of BC005386 Sequence: MKLLVLAVLLTVAAADSGISPRAVWQFRKMIKCVIPGSDPFLEYNNYGCYCGLGGSGTPVDELDKQKQRV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phospholipase A2, group IB (pancreas) |
| 别名 | PLA2, Phosphatidylcholine 2-acylhydrolase 1B, EC:3.1.1.4 |
| 计算分子量 | 70aa,8 kDa; 148aa,16 kDa |
| 观测分子量 | 13-16 kDa |
| GenBank蛋白编号 | BC005386 |
| 基因名称 | PLA2G1B |
| Gene ID (NCBI) | 5319 |
| RRID | AB_2878190 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P04054 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLA2G1B is a secreted member of the phospholipase A2 (PLA2) class of enzymes, which is produced by the pancreatic acinar cells. PLA2G1B catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to release arachidonic acid (AA) and lysophospholipids.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for PLA2G1B antibody 15843-1-AP | Download protocol |
| IHC protocol for PLA2G1B antibody 15843-1-AP | Download protocol |
| WB protocol for PLA2G1B antibody 15843-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep Identification and verification of biomarkers associated with arachidonic acid metabolism in non-alcoholic fatty liver disease | ||
Food Chem Dietary sn-2 palmitate influences cognitive behavior by increasing the transport of liver-produced lysophosphatidylcholine VLCPUFAs to the brain |
