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验证数据展示

  • WB analysis of mouse skeletal muscle using 16009-1-AP (same clone as 16009-1-PBS)

    WB analysis of mouse skeletal muscle using 16009-1-AP (same clone as 16009-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16009-1-AP (PLA2G12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16009-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 16009-1-AP (same clone as 16009-1-PBS)

    IHC staining of human colon cancer using 16009-1-AP (same clone as 16009-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 16009-1-AP (PLA2G12A antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16009-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 16009-1-AP (same clone as 16009-1-PBS)

    IHC staining of human colon cancer using 16009-1-AP (same clone as 16009-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 16009-1-AP (PLA2G12A antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16009-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 16009-1-AP (same clone as 16009-1-PBS)

    IF Staining of HEK-293 using 16009-1-AP (same clone as 16009-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 16009-1-AP (PLA2G12A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16009-1-PBS in a different storage buffer formulation.

PLA2G12A Polyclonal antibody, PBS Only

PLA2G12A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 16009-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16009-1-PBS targets PLA2G12A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8722

Product name: Recombinant human PLA2G12A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 23-189 aa of BC017218

Sequence: QEQAQTTDWRATLKTIRNGVHKIDTYLNAALDLLGGEDGLCQYKCSDGSKPFPRYGYKPSPPNGCGSPLFGVHLNIGIPSLTKCCNQHDRCYETCGKSKNDCDEEFQYCLSKICRDVQKTLGLTQHVQACETTVELLFDSVIHLGCKPYLDSQRAACRCHYEEKTDL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase A2, group XIIA
别名
计算分子量 189 aa, 21 kDa
观测分子量 21-24 kDa
GenBank蛋白编号BC017218
基因名称 PLA2G12A
Gene ID (NCBI) 81579
RRIDAB_2164314
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BZM1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLA2G12A (or PLA2G12) gene encodes group XII secreted phospholipase A2 (sPLA2) which catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Cellular arachidonate (AA) release and prostaglandin (PG) production is regulated by sPLA(2)s, groups III and XII. Group XII sPLA2 have relatively low specific activity and are structurally and functionally distinct from other sPLA2s. Cells transfected with group XII sPLA(2) exhibited abnormal morphology, suggesting a unique functional aspect of this enzyme. Role of sPLA2s as potential tumour biomarkers and therapeutic targets for various cancers have been reported.

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