验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, Calu-1 cells, Calu-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19502-1-AP targets PLA2G10 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
fusion protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phospholipase A2, group X |
| 别名 | EC:3.1.1.4, GX sPLA2, GXPLA2, GXSPLA2, Phosphatidylcholine 2-acylhydrolase 10 |
| 计算分子量 | 18 kDa |
| 观测分子量 | 18-20 kDa |
| GenBank蛋白编号 | NM_003561 |
| 基因名称 | PLA2G10 |
| Gene ID (NCBI) | 8399 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15496 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PLA2G10 belongs to the phospholipase A2 family. PLA2G10 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. PLA2G10 has a powerful potency for releasing arachidonic acid from cell membrane phospholipids.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLA2G10 antibody 19502-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |