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验证数据展示

  • WB analysis of K-562 using 18338-1-AP

    WB analysis of K-562 using 18338-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of A549 using 18338-1-AP

    IP experiment of A549 using 18338-1-AP

    IP result of anti-Plakophilin 3 (IP:18338-1-AP, 4ug; Detection:18338-1-AP 1:600) with A549 cells lysate 1000ug.

  • IHC staining of human skin using 18338-1-AP

    IHC staining of human skin using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18338-1-AP (Plakophilin 3 antibody at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with Plakophilin 3 antibody included
  • IHC staining of human skin using 18338-1-AP

    IHC staining of human skin using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 18338-1-AP (Plakophilin 3 antibody at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with Plakophilin 3 antibody included
  • IHC staining of mouse skin using 18338-1-AP

    IHC staining of mouse skin using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Plakophilin 3 antibody included
  • IHC staining of human placenta using 18338-1-AP

    IHC staining of human placenta using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Plakophilin 3 antibody included
  • IHC staining of human stomach cancer using 18338-1-AP

    IHC staining of human stomach cancer using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Plakophilin 3 antibody included
  • IHC staining of mouse cerebellum using 18338-1-AP

    IHC staining of mouse cerebellum using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Plakophilin 3 antibody included
  • IHC staining of rat cerebellum using 18338-1-AP

    IHC staining of rat cerebellum using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Plakophilin 3 antibody included
  • IHC staining of rat skin using 18338-1-AP

    IHC staining of rat skin using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Plakophilin 3 antibody included
  • IHC staining of human placenta using 18338-1-AP

    IHC staining of human placenta using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Plakophilin 3 antibody included
  • IHC staining of human colon using 18338-1-AP

    IHC staining of human colon using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Plakophilin 3 antibody included
  • IHC staining of human bowen disease using 18338-1-AP

    IHC staining of human bowen disease using 18338-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 18338-1-AP (Plakophilin 3 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Plakophilin 3 antibody included

Plakophilin 3 Polyclonal antibody

Plakophilin 3 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 18338-1-AP

产品说明书

引用文献(5)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

PKP3, Plakophilin-3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, A549 cells, HeLa cells, MCF-7 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman skin tissue, human placenta tissue, human colon tissue, human stomach cancer tissue, human bowen disease tissue, mouse skin tissue, rat cerebellum tissue, rat skin tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy® Plakophilin 3 Ready-To-Use IHC Kit. Plakophilin 3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18338-1-AP targets Plakophilin 3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag13062

Product name: Recombinant human PKP3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 170-400 aa of BC000081

Sequence: VGSRADYDTLSLRSLRLGPGGLDDRYSLVSEQLEPAATSTYRAFAYERQASSSSSRAGGLDWPEATEVSPSRTIRAPAVRTLQRFQSSHRSRGVGGAVPGAVLEPVARAPSVRSLSLSLADSGHLPDVHGFNSYGSHRTLQRLSSGFDDIDLPSAVKYLMASDPNLQVLGAAYIQHKCYSDAAAKKQARSLQAVPRLVKLFNHANQEVQRHATGAMRNLIYDNADNKLALV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 plakophilin 3
别名 PKP3, Plakophilin-3
计算分子量 87 kDa
观测分子量 87 kDa
GenBank蛋白编号BC000081
基因名称 Plakophilin 3
Gene ID (NCBI) 11187
RRIDAB_2164281
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y446
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for Plakophilin 3 antibody 18338-1-APDownload protocol
IP protocol for Plakophilin 3 antibody 18338-1-APDownload protocol
WB protocol for Plakophilin 3 antibody 18338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,humanWB,IHC

Cell Rep

Plakophilin 3 drives acinar cell transformation and promotes cancer initiation and progression in pancreas.

Authors - Xiaojia Li
  • KO Validated
View Article
ratWB,IHC

J Ovarian Res

Cangfu Daotan Wan alleviates polycystic ovary syndrome with phlegm-dampness syndrome via disruption of the PKP3/ERCC1/MAPK axis

Authors - Yuan Li
View Article
humanWB

Sci Rep

SHP2 improves ovarian morphology and steroidogenic function in a rat PCOS model by modulating IRE1α/XBP1/NLRP3-mediated granulosa cell pyroptosis.

Authors - DongXue Wang
View Article
humanIHC

Med Oncol

Data-independent acquisition (DIA) mass spectrometry reveals related proteins involved in the occurrence of early intestinal-type gastric cancer

Authors - Liangshun Zhang
View Article
humanIHC

Biomedicines

Comprehensive Analysis Identifies PKP3 Overexpression in Pancreatic Cancer Related to Unfavorable Prognosis

Authors - Yan Du
View Article
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