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PKP1 Polyclonal antibody

PKP1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 22632-1-AP

Print datasheet

Synonyms

B6P, Band 6 protein, PKP1, Plakophilin 1

验证数据展示

  • WB analysis using 22632-1-AP

    WB analysis using 22632-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22632-1-AP (PKP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHaCaT cells, mouse skin tissue, rat skin tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22632-1-AP targets PKP1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen PKP1 fusion protein Ag18153 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name plakophilin 1 (ectodermal dysplasia/skin fragility syndrome)
Synonyms B6P, Band 6 protein, PKP1, Plakophilin 1
Calculated Molecular Weight 747 aa, 83 kDa
Observed Molecular Weight70-75 kDa
GenBank Accession NumberBC114571
Gene Symbol PKP1
Gene ID (NCBI) 5317
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13835
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Plakophilin 1, also known as PKP1, B6P, is a member of the arm-repeat protein family. PKP1 contains arm-repeat (armadillo) domains, localizes to nuclei and cell desmosomes, and participates in linking cadherins to intermediate filaments in the cytoskeleton (PMID: 10747098). PKP1 contributes to epidermal morphogenesis and facilitates the formation of intermediate filaments. Mutations in PKP1 have been associated with the ectodermal dysplasia/skin fragility syndrome (PubMed:9326952, 10852826).

实验方案

Product Specific Protocols
WB protocol for PKP1 antibody 22632-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols