验证数据展示
经过测试的应用
| Positive WB detected in | RAW 264.7 cells, RAW264.7 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20352-1-AP targets PKNOX2 in WB, IF, RIP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, RIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse, pig |
| 免疫原 |
CatNo: Ag14195 Product name: Recombinant human PKNOX2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 350-471 aa of BC045626 Sequence: MLDASNPDPAPKAKKIKSQHRPTQRFWPNSIAAGVLQQQGGAPGTNPDGSINLDNLQSLSSDSATMAMQQAMMAAHDDSLDGTEEEDEDEMEEEEEELEEEVDELQTTNVSDLGLEHSDSLE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | PBX/knotted 1 homeobox 2 |
| 别名 | Homeobox protein PKNOX2, Homeobox protein PREP 2, PBX/knotted 1 homeobox 2, PBX/knotted homeobox 2, PKNOX2, PREP2 |
| 计算分子量 | 472 aa, 52 kDa |
| 观测分子量 | 70 kDa, 55 kDa |
| GenBank蛋白编号 | BC045626 |
| 基因名称 | PKNOX2 |
| Gene ID (NCBI) | 63876 |
| RRID | AB_10694277 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96KN3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PKNOX2, PBX/knotted 1 homeobox 2, also named as PREP2, belongs to the three-amino acid loop extension (TALE) homeobox family. As it contains a DNA-binding motif, PKNOX2 may function as a nuclear transcription factor, experiments have reported the PKNOX2 protein locates to the nucleus. It was showed that highest expression of a 4-kb PREP2 (PKNOX2) transcript in heart, brain, skeletal muscle, and ovary(PMID:11972344). Several years later, PKNOX2 was identified as one of the cis-regulated genes for alcohol addiction in mice, however it has not been reported to be associated with any similar phenotype in humans to date (PMID:19721000). The molecular weight of the protein may differ from the theoretical value.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for PKNOX2 antibody 20352-1-AP | Download protocol |
| WB protocol for PKNOX2 antibody 20352-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun PKNOX2 regulates myofibroblast functions and tubular cell survival during kidney fibrosis. | ||
Int J Mol Sci LncRNA TCONS_00323213 Promotes Myogenic Differentiation by Interacting with PKNOX2 to Upregulate MyoG in Porcine Satellite Cells |
