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PKNOX1 Polyclonal antibody

PKNOX1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10614-1-AP

Print datasheet

Synonyms

Homeobox protein PKNOX1, Homeobox protein PREP 1, PBX/knotted 1 homeobox 1, PBX/knotted homeobox 1, PKNOX1, pkonx1c, PREP1

验证数据展示

  • IHC staining of human gliomas using 10614-1-AP

    IHC staining of human gliomas using 10614-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas using 10614-1-AP (PKNOX1 antibody) at dilution of 1:100 (under 25x lens).

  • WB analysis of SH-SY5Y using 10614-1-AP

    WB analysis of SH-SY5Y using 10614-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10614-1-AP (PKNOX1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 10614-1-AP

    IF Staining of HepG2 using 10614-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10614-1-AP (PKNOX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inSH-SY5Y cells, COLO 320 cells, HepG2 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10614-1-AP targets PKNOX1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IP, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen PKNOX1 fusion protein Ag0939 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name PBX/knotted 1 homeobox 1
Synonyms Homeobox protein PKNOX1, Homeobox protein PREP 1, PBX/knotted 1 homeobox 1, PBX/knotted homeobox 1, PKNOX1, pkonx1c, PREP1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC007746
Gene Symbol PKNOX1
Gene ID (NCBI) 5316
RRIDAB_2164125
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55347
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PKNOX1 antibody 10614-1-APDownload protocol
IHC protocol for PKNOX1 antibody 10614-1-APDownload protocol
IF protocol for PKNOX1 antibody 10614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

Am J Hum Genet

Gain-of-Function MN1 Truncation Variants Cause a Recognizable Syndrome with Craniofacial and Brain Abnormalities.

Authors - Noriko Miyake
View Article
mouseWB,IHC

Am J Physiol Renal Physiol

PMID: 31091119

Authors - Zhiqiang Chen
View Article