验证数据展示
产品信息
84451-1-PBS targets PKM2-specific in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description |
经测试反应性 | human |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | PKM pyruvate kinase, muscle |
别名 | PKM, Pyruvate kinase PKM, EC:2.7.11.1, EC:2.7.10.2, EC:2.7.1.40 |
计算分子量 | 58 kDa |
观测分子量 | 58 kDa |
GenBank蛋白编号 | NM_002654 |
基因名称 | PKM |
Gene ID (NCBI) | 5315 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | P14618 |
储存缓冲液 | PBS only , pH 7.3 |
储存条件 | Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C |
背景介绍
PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.