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- KD/KO Validated
PKM2-specific Polyclonal antibody
PKM2-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
IF, IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
162
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HeLa cells, HEK-293 cells, MCF-7 cells, Recombinant protein, mouse brain tissue, N/A, HEK-293T cells, mouse stomach tissue |
Positive IP detected in | A549 cells, MCF-7 cells |
Positive IHC detected in | human lung cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15822-1-AP targets PKM2-specific in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF, IHC, IP, WB, ELISA |
Cited Applications | ChIP, CoIP, IF, IHC, IP, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken, bovine |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | PKM pyruvate kinase, muscle |
Synonyms | CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM2, PK-M2, PKM2 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase isozymes M1/M2, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK |
Calculated Molecular Weight | 58 kDa |
Observed Molecular Weight | 58 kDa |
GenBank Accession Number | NM_002654 |
Gene Symbol | PKM |
Gene ID (NCBI) | 5315 |
RRID | AB_1851537 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P14618 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for PKM2-specific antibody 15822-1-AP | Download protocol |
IHC protocol for PKM2-specific antibody 15822-1-AP | Download protocol |
IF protocol for PKM2-specific antibody 15822-1-AP | Download protocol |
IP protocol for PKM2-specific antibody 15822-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nature The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth.
| ||
Mol Cell The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis. | ||
Biomaterials PKM2 allosteric converter: A self-assembly peptide for suppressing renal cell carcinoma and sensitizing chemotherapy | ||
Nat Commun Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2. | ||
Clin Transl Med USP7 promotes non-small-cell lung cancer cell glycolysis and survival by stabilizing and activating c-Abl |