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PKLR Polyclonal antibody

PKLR Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 17580-1-AP

Print datasheet

Synonyms

PK1, PKL, PKLR, PKR, PKRL, Pyruvate kinase 1, Pyruvate kinase isozymes R/L, pyruvate kinase, liver and RBC, R type/L type pyruvate kinase, Red cell/liver pyruvate kinase, RPK

验证数据展示

  • WB analysis of K-562 using 17580-1-AP

    WB analysis of K-562 using 17580-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 17580-1-AP (PKLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 17580-1-AP

    IHC staining of human liver using 17580-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17580-1-AP (PKLR antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with PKLR antibody included
  • IHC staining of human liver using 17580-1-AP

    IHC staining of human liver using 17580-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17580-1-AP (PKLR antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with PKLR antibody included
  • IP experiment of K-562 using 17580-1-AP

    IP experiment of K-562 using 17580-1-AP

    IP result of anti-PKLR (IP:17580-1-AP, 4ug; Detection:17580-1-AP 1:500) with K-562 cells lysate 3200ug.

经过测试的应用

Positive WB detected inK-562 cells, mouse kidney tissue, mouse liver tissue, NIH/3T3 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PKLR Ready-To-Use IHC Kit. PKLR primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17580-1-AP targets PKLR in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen PKLR fusion protein Ag11096 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name pyruvate kinase, liver and RBC
Synonyms PK1, PKL, PKLR, PKR, PKRL, Pyruvate kinase 1, Pyruvate kinase isozymes R/L, pyruvate kinase, liver and RBC, R type/L type pyruvate kinase, Red cell/liver pyruvate kinase, RPK
Calculated Molecular Weight 574 aa, 62 kDa, 585 aa, 63 kDa
Observed Molecular Weight 58-62 kDa
GenBank Accession NumberBC025737
Gene Symbol PKLR
Gene ID (NCBI) 5313
RRIDAB_2268201
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30613
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PKLR antibody 17580-1-APDownload protocol
IHC protocol for PKLR antibody 17580-1-APDownload protocol
IP protocol for PKLR antibody 17580-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols