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PKIB Polyclonal antibody

PKIB Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat No : 11942-1-AP

Print datasheet

Synonyms

PKI beta, PKIB, PRKACN2

验证数据展示

  • IHC staining of human stomach cancer using 11942-1-AP

    IHC staining of human stomach cancer using 11942-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11942-1-AP (PKIB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 11942-1-AP

    IHC staining of human stomach cancer using 11942-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11942-1-AP (PKIB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inhuman stomach cancer tissue, human gliomas tissue, mouse kidney tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11942-1-AP targets PKIB in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, ELISA
Tested Reactivity human, mouse
Immunogen PKIB fusion protein Ag2541 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase (cAMP-dependent, catalytic) inhibitor beta
Synonyms PKI beta, PKIB, PRKACN2
Calculated Molecular Weight 78 aa, 9 kDa
GenBank Accession NumberBC036011
Gene Symbol PKIB
Gene ID (NCBI) 5570
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9C010
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The cAMP-dependent protein kinase inhibitor-β (PKIB) is also named as PRKACN2. PKIB is presumed to be one of the regulatory factors controlling the cAMP-dependent protein kinase A signaling pathway (PMID: 23224602). PKIB plays an important role in regulating the proliferation, migration, and even metastasis of osteosarcoma (PMID: 17195088). The expression of PKIB in the cytoplasm of tumor is closely related to pAkt and the triple negative breast cancer (PMID: 28387904).

实验方案

Product Specific Protocols
IHC protocol for PKIB antibody 11942-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols