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验证数据展示

  • IHC staining of mouse embryo using 11743-1-AP

    IHC staining of mouse embryo using 11743-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11743-1-AP (PKIA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PKIA antibody included
  • IHC staining of mouse embryo using 11743-1-AP

    IHC staining of mouse embryo using 11743-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11743-1-AP (PKIA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PKIA antibody included
  • IHC staining of mouse brain using 11743-1-AP

    IHC staining of mouse brain using 11743-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11743-1-AP (PKIA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PKIA antibody included
  • IHC staining of mouse brain using 11743-1-AP

    IHC staining of mouse brain using 11743-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11743-1-AP (PKIA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PKIA antibody included
  • IF Staining of HeLa using 11743-1-AP

    IF Staining of HeLa using 11743-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PKIA antibody (11743-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

PKIA Polyclonal antibody

PKIA Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 11743-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, IF/ICC, ELISA

别名

PRKACN1, PKI-alpha, PKI alpha, cAMP-dependent protein kinase inhibitor, muscle/brain isoform, cAMP-dependent protein kinase inhibitor alpha

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IHC detected inmouse embryo tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy PKIA Ready-To-Use IHC Kit. PKIA primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11743-1-AP targets PKIA in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2338

Product name: Recombinant human PKIA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-76 aa of BC022265

Sequence: MTDVETTYADFIASGRTGRRNAIHDILVSSASGNSNELALKLAGLDINKTEGEEDAQRSSTEQSGEAQGEAAKSES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase (cAMP-dependent, catalytic) inhibitor alpha
别名 PRKACN1, PKI-alpha, PKI alpha, cAMP-dependent protein kinase inhibitor, muscle/brain isoform, cAMP-dependent protein kinase inhibitor alpha
计算分子量 76 aa, 8 kDa
GenBank蛋白编号BC022265
基因名称 PKIA
Gene ID (NCBI) 5569
RRIDAB_3085380
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP61925
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

cAMP-dependent protein kinase inhibitor alpha (PKIA) is also named as PRKACN1, and belongs to the PKI family. Among them, PKIA, a member of protein kinase A (PKA) inhibitor family, was found to be most significantly and highly expressed in susceptible animals (PMID:24910983). Decreased PKIA signaling would directly impact PKA activation, potentially inducing DRP1 phosphorylation and increasing ER Ca2+ stores (PMID:32152556). miR-129-5p activated PKA to regulate the phosphorylation of beta-catenin and cAMP-response element binding protein (CREB) by inhibiting PKIA (PMID:36222334).

实验方案

Product Specific Protocols
IF protocol for PKIA antibody 11743-1-APDownload protocol
IHC protocol for PKIA antibody 11743-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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