• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PKIA Polyclonal antibody

PKIA Polyclonal Antibody for IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 11743-1-AP

Print datasheet

Synonyms

PKI alpha, PKIA, PRKACN1

验证数据展示

  • IHC staining of mouse embryo using 11743-1-AP

    IHC staining of mouse embryo using 11743-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11743-1-AP (PKIA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse embryo using 11743-1-AP

    IHC staining of mouse embryo using 11743-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 11743-1-AP (PKIA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 11743-1-AP

    IHC staining of mouse brain using 11743-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11743-1-AP (PKIA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 11743-1-AP

    IHC staining of mouse brain using 11743-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11743-1-AP (PKIA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 11743-1-AP

    IF Staining of HeLa using 11743-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PKIA antibody (11743-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inmouse embryo tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11743-1-AP targets PKIA in IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, ELISA
Tested Reactivity human, mouse, rat
Immunogen PKIA fusion protein Ag2338 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase (cAMP-dependent, catalytic) inhibitor alpha
Synonyms PKI alpha, PKIA, PRKACN1
Calculated Molecular Weight 76 aa, 8 kDa
GenBank Accession NumberBC022265
Gene Symbol PKIA
Gene ID (NCBI) 5569
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61925
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

cAMP-dependent protein kinase inhibitor alpha (PKIA) is also named as PRKACN1, and belongs to the PKI family. Among them, PKIA, a member of protein kinase A (PKA) inhibitor family, was found to be most significantly and highly expressed in susceptible animals (PMID:24910983). Decreased PKIA signaling would directly impact PKA activation, potentially inducing DRP1 phosphorylation and increasing ER Ca2+ stores (PMID:32152556). miR-129-5p activated PKA to regulate the phosphorylation of beta-catenin and cAMP-response element binding protein (CREB) by inhibiting PKIA (PMID:36222334).

实验方案

Product Specific Protocols
IHC protocol for PKIA antibody 11743-1-APDownload protocol
IF protocol for PKIA antibody 11743-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols