验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16333-1-AP targets PKD1L2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag9455 Product name: Recombinant human PKD1L2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-306 aa of BC014157 Sequence: MGEDSPVAMFSWYLDNTPTEQAEPLPDACRLRGFWPRSLTLLQSNTSTLLLNSSFLQSRGEVIRIRATALTRHAYGEDTYVISTVPPREVPACTIAPEEGTVLTSFAIFCNASTALGPLEFCFCLESGSCLHCGPEPALPSVYLPLGEENNDFVLTVVISATNRAGDTQQTQAMAKVALGDTCVEDVAFQAAVSEKIPTALQGEGGPEQLLQLAKAVSSMLNQEHESQGSGQSLSIDVRQKVREHVLGSLSAVTTGLEDVQRVQELAEVLREVTCRSKELTPSAQGSCMGDSWEGAPPAAHVSHAR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | polycystic kidney disease 1-like 2 |
| 别名 | Polycystin-1L2, Polycystin 1L2, Polycystic kidney disease protein 1-like 2, PC1-like 2 protein, PC1L2 |
| 计算分子量 | 306aa,33 kDa; 2459aa,273 kDa |
| 观测分子量 | 270 kDa |
| GenBank蛋白编号 | BC014157 |
| 基因名称 | PKD1L2 |
| Gene ID (NCBI) | 114780 |
| RRID | AB_3669239 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z442 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PKD1L2 is a member of the polycystin protein family. PKD1L2 may function as a G-protein-coupled component or regulator of cation channel pores. The long isoform of this protein contains 11 transmembrane domains, a latrophilin/CL-1-like GPCR proteolytic site (GPS) domain, and a polycystin-1, lipoxygenase, alpha-toxin (PLAT) domain.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PKD1L2 antibody 16333-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
