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验证数据展示

  • WB analysis using 85733-2-RR (same clone as 85733-2-PBS)

    WB analysis using 85733-2-RR (same clone as 85733-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85733-2-RR (PRKCI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85733-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02074-3

    Sandwich ELISA sample test of MP02074-3

    The mean PKC Iota concentration was determined to be 47.20 ng/mL in HeLa cell extract based on a 3.0 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02074-3

    Sandwich ELISA standard curve of MP02074-3

    Sandwich ELISA standard curve of MP02074-3, Human PKC Iota Recombinant Matched Antibody Pair - PBS only. 85733-6-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4990. 85733-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

  • IF Staining of HEK-293T using 85733-2-RR (same clone as 85733-2-PBS)

    IF Staining of HEK-293T using 85733-2-RR (same clone as 85733-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293T cells using PKC Iota antibody (85733-2-RR, Clone: 243126A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85733-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85733-2-RR

    Affinity and Kinetic Characterization of 85733-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85733-2-RR against Human PKC Iota were performed. The affinity constant is below 1 pM.

PKC Iota Recombinant monoclonal antibody, PBS Only (Detector)

PKC Iota Uni-rAb® Recombinant Antibody for WB, IF/ICC, Sandwich ELISA, Indirect ELISA
Cat No. 85733-2-PBS

产品说明书

CloneNo. 243126A4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Sandwich ELISA, Indirect ELISA

别名

PRKCI, aPKC lambda/iota, aPKC-lambda/iota, Atypical protein kinase C-lambda/iota, DXS1179E

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PRKCI Ready-To-Use IHC Kit. PRKCI primary antibody included.

产品信息

85733-2-PBS targets PKC Iota as part of a matched antibody pair:

MP02074-3: 85733-6-PBS capture and 85733-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4990

Product name: Recombinant human PRKCI protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 238-587 aa of BC022016

Sequence: SSLGLQDFDLLRVIGRGSYAKVLLVRLKKTDRIYAMKVVKKELVNDDEDIDWVQTEKHVFEQASNHPFLVGLHSCFQTESRLFFVIEYVNGGDLMFHMQRQRKLPEEHARFYSAEISLALNYLHERGIIYRDLKLDNVLLDSEGHIKLTDYGMCKEGLRPGDTTSTFCGTPNYIAPEILRGEDYGFSVDWWALGVLMFEMMAGRSPFDIVGSSDNPDQNTEDYLFQVILEKQIRIPRSMSVKAASVLKSFLNKDPKERLGCLPQTGFADIQGHPFFRNVDWDMMEQKQVVPPFKPNISGEFGLDNFDSQFTNERVQLTPDDDDIVRKIDQSEFEGFEYINPLLMSAEECV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein kinase C, iota
别名 PRKCI, aPKC lambda/iota, aPKC-lambda/iota, Atypical protein kinase C-lambda/iota, DXS1179E
计算分子量 68 kDa
观测分子量 67 kDa
GenBank蛋白编号BC022016
基因名称 PKC Iota
Gene ID (NCBI) 5584
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP41743
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The atypical protein kinase C isoform PRKC iota (PRKCI) is a member of the protein kinase C (PKC) family of serine/threonine protein kinases. PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. PRKC iota is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. PRKC iota plays a key role in cell proliferation, differentiation, and carcinogenesis, and it has been shown to be a human oncogene.

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