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验证数据展示

  • WB analysis using 19132-1-AP (same clone as 19132-1-PBS)

    WB analysis using 19132-1-AP (same clone as 19132-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19132-1-AP (PKC Delta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

  • IP experiment of A431 using 19132-1-AP (same clone as 19132-1-PBS)

    IP experiment of A431 using 19132-1-AP (same clone as 19132-1-PBS)

    IP result of anti-PKC Delta (IP:19132-1-AP, 4ug; Detection:19132-1-AP 1:800) with A431 cells lysate 2640 ug. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

  • IP experiment of A431 using 19132-1-AP (same clone as 19132-1-PBS)

    IP experiment of A431 using 19132-1-AP (same clone as 19132-1-PBS)

    IP result of anti-PKC Delta (IP:19132-1-AP, 4ug; Detection:19132-1-AP 1:500) with A431 cells lysate 2000ug. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 19132-1-AP (same clone as 19132-1-PBS)

    IF Staining of U2OS using 19132-1-AP (same clone as 19132-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 19132-1-AP (same clone as 19132-1-PBS)

    FC experiment of HeLa using 19132-1-AP (same clone as 19132-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PKC Delta (19132-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

  • IF Staining of U-251 using 19132-1-AP (same clone as 19132-1-PBS)

    IF Staining of U-251 using 19132-1-AP (same clone as 19132-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 19132-1-AP (same clone as 19132-1-PBS)

    IF Staining of HeLa using 19132-1-AP (same clone as 19132-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKC Delta antibody (19132-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

  • WB analysis using 19132-1-AP (same clone as 19132-1-PBS)

    WB analysis using 19132-1-AP (same clone as 19132-1-PBS)

    Non-treated and PMA treated THP-1 cells were subjected to SDS PAGE followed by western blot with 29564-1-AP (Phospho-PKC delta (Thr507) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with PKC Delta antibody (19132-1-AP) subsequently. This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 19132-1-AP (same clone as 19132-1-PBS)

    IHC staining of mouse liver using 19132-1-AP (same clone as 19132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19132-1-AP (PKC Delta antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKCD antibody included
  • IHC staining of mouse liver using 19132-1-AP (same clone as 19132-1-PBS)

    IHC staining of mouse liver using 19132-1-AP (same clone as 19132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 19132-1-AP (PKC Delta antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19132-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PRKCD antibody included
  • Featured Product
  • KD/KO Validated

PKC Delta Polyclonal antibody, PBS Only

PKC Delta Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 19132-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

PRKCD, EC:2.7.10.2, EC:2.7.11.13, MAY1, nPKC-delta

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PRKCD Ready-To-Use IHC Kit. PRKCD primary antibody included.

产品信息

19132-1-PBS targets PKC Delta in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5631

Product name: Recombinant human PRKCD protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 326-676 aa of BC043350

Sequence: DMQDNSGTYGKIWEGSSKCNINNFIFHKVLGKGSFGKVLLGELKGRGEYFAIKALKKDVVLIDDDVECTMVEKRVLTLAAENPFLTHLICTFQTKDHLFFVMEFLNGGDLMYHIQDKGRFELYRATFYAAEIMCGLQFLHSKGIIYRDLKLDNVLLDRDGHIKIADFGMCKENIFGESRASTFCGTPDYIAPEILQGLKYTFSVDWWSFGVLLYEMLIGQSPFHGDDEDELFESIRVDTPHYPRWITKESKDILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase C, delta
别名 PRKCD, EC:2.7.10.2, EC:2.7.11.13, MAY1, nPKC-delta
计算分子量 78 kDa
观测分子量 70 kDa
GenBank蛋白编号BC043350
基因名称 PKC Delta
Gene ID (NCBI) 5580
RRIDAB_10637263
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ05655
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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