- Featured Product
- KD/KO Validated
PJA1 Polyclonal antibody
PJA1 Polyclonal Antibody for IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IP, WB,ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17687-1-AP targets PJA1 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IP, WB,ELISA |
| Cited Applications | WB, IF, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Immunogen | PJA1 fusion protein Ag11820 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | praja ring finger 1 |
| Synonyms | PJA1, praja ring finger 1, Praja1, RING finger protein 70, RNF70 |
| Calculated Molecular Weight | 643 aa, 71 kDa |
| Observed Molecular Weight | 71-80 kDa |
| GenBank Accession Number | BC075803 |
| Gene Symbol | PJA1 |
| Gene ID (NCBI) | 64219 |
| RRID | AB_2252483 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NG27 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PJA1(E3 ubiquitin-protein ligase Praja-1), also named as RNF70, regulates the level of PRC2 (polycomb repressive complex 2) by targeting its free subunits for Ub-mediated proteasomal degradation and it can be involved in protein ubiquitination in the brain and is a suitable candidate gene for MRX(PMID:12036302). The Praja1 protein is unlikely to be a membrane receptor, since it lacks a hydrophobic transmembrane domain(PMID:9393880). It has 3 isoforms produced by alternative splicing with the molecular weight of 71 kDa, 50 kDa and 65 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PJA1 antibody 17687-1-AP | Download protocol |
| IP protocol for PJA1 antibody 17687-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Praja1 E3 ubiquitin ligase promotes skeletal myogenesis through degradation of EZH2 upon p38α activation.
| ||
Ann Clin Transl Neurol A recurrent PJA1 variant in trigonocephaly and neurodevelopmental disorders. | ||
Biochim Biophys Acta Mol Cell Biol Lipids Diacylglycerol kinase δ destabilizes serotonin transporter protein through the ubiquitin-proteasome system. | ||
J Virol PJA1 Coordinates with the SMC5/6 Complex to Restrict DNA Viruses and Episomal Genes through Interferon-independent Manner. | ||
Neuropathology Praja1 RING-finger E3 ubiquitin ligase suppresses neuronal cytoplasmic TDP-43 aggregate formation. | ||
Neuropathology Praja1 RING-finger E3 ubiquitin ligase is a common suppressor of neurodegenerative disease-associated protein aggregation. |
