验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue, mouse testis tissue, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12966-1-AP targets PIWIL4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag3613 Product name: Recombinant human PIWIL4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 544-852 aa of BC031060 Sequence: VQLVMCILPSNQKTYYDSIKKYLSSDCPVPSQCVLARTLNKQGMMMSIATKIAMQMTCKLGGELWAVEIPLKSLMVVGIDVCKDALSKDVMVVGCVASVNPRITRWFSRCILQRTMTDVADCLKVFMTGALNKWYKYNHDLPARIIVYRAGVGDGQLKTLIEYEVPQLLSSVAESSSNTSSRLSVIVVRKKCMPRFFTEMNRTVQNPPLGTVVDSEATRNEWYDFYLISQVACRGTVSPTYYNVIYDDNGLKPDHMQRLTFKLCHLYYNWPGIVSVPAPCQYAHKLTFLVAQSIHKEPSLELANHLFYL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | piwi-like 4 (Drosophila) |
| 别名 | HIWI2, MIWI2, PIWI, piwi like 4 (Drosophila), Piwi like protein 4 |
| 计算分子量 | 852 aa, 97 kDa |
| 观测分子量 | 97 kDa |
| GenBank蛋白编号 | BC031060 |
| 基因名称 | PIWIL4 |
| Gene ID (NCBI) | 143689 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7Z3Z4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PIWIL4 (PiWI-like RNA-mediated gene silencing 4) is a member of the PIWI protein family and is mainly involved in the development and maintenance of reproductive stem cells. PIWIL4 regulates the biosynthesis and function of piRNA in germ cells by binding to PIWI-interacting RNA (piRNA). Furthermore, PIWIL4 is abnormally expressed in a variety of cancers and plays a promoting role in cancer.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PIWIL4 antibody 12966-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
