PIWIL1 Polyclonal antibody

PIWIL1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 15659-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA and More (1)

Piwi like protein 1, piwi like 1 (Drosophila), PIWI, MIWI, HIWI

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inhuman testis tissue, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman testis tissue, human liver cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15659-1-AP targets PIWIL1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human, mouse
文献引用反应性human, mouse, chicken
免疫原 PIWIL1 fusion protein Ag8056 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 piwi-like 1 (Drosophila)
别名 Piwi like protein 1, piwi like 1 (Drosophila), PIWI, MIWI, HIWI
计算分子量 99 kDa
观测分子量 85-95 kDa
GenBank蛋白编号BC028581
基因名称 PIWIL1
Gene ID (NCBI) 9271
RRIDAB_2878163
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96J94
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIWIL1 antibody 15659-1-APDownload protocol
IHC protocol for PIWIL1 antibody 15659-1-APDownload protocol
IF protocol for PIWIL1 antibody 15659-1-APDownload protocol
IP protocol for PIWIL1 antibody 15659-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF,IP

Development

MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis

Authors -
humanWB

Mol Ther Nucleic Acids

PIWIL1/piRNA-DQ593109 Regulates the Permeability of the Blood-Tumor Barrier via the MEG3/miR-330-5p/RUNX3 Axis.

Authors - Shuyuan Shen
mouseWB,IF,IP

Development

MFN2 interacts with nuage-associated proteins and is essential for male germ cell development by controlling mRNA fate during spermatogenesis.

Authors - Xiaoli Wang
humanWB

J Transl Med

piRNA-14633 promotes cervical cancer cell malignancy in a METTL14-dependent m6A RNA methylation manner.

Authors - Qi Xie
humanIF

Prostate

High levels of PIWI-interacting RNAs are present in the small RNA landscape of prostate epithelium from vitamin D clinical trial specimens.

Authors - Bethany Baumann
mouseWB

Andrologia

Secretions released from mesenchymal stem cells improve spermatogenesis restoration of cytotoxic treatment with busulfan in azoospermia mice.

Authors - Yi-Ting Cai
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