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验证数据展示

  • WB analysis using 30674-1-AP (same clone as 30674-1-PBS)

    WB analysis using 30674-1-AP (same clone as 30674-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30674-1-AP (PITRM1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30674-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 30674-1-AP (same clone as 30674-1-PBS)

    IHC staining of human breast cancer using 30674-1-AP (same clone as 30674-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 30674-1-AP (PITRM1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30674-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PITRM1 antibody included
  • IHC staining of human breast cancer using 30674-1-AP (same clone as 30674-1-PBS)

    IHC staining of human breast cancer using 30674-1-AP (same clone as 30674-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 30674-1-AP (PITRM1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30674-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PITRM1 antibody included

PITRM1 Polyclonal antibody, PBS Only

PITRM1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 30674-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PITRM1 Ready-To-Use IHC Kit. PITRM1 primary antibody included.

产品信息

30674-1-PBS targets PITRM1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30478

Product name: Recombinant human PITRM1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 258-410 aa of BC001150

Sequence: ILRKLPRIKKHLLNGDNMRCSVNATPQQMPQTEKAVEDFLRSIGRSKKERRPVRPHTVEKPVPSSSGGDAHVPHGSQVIRKLVMEPTFKPWQMKTHFLMPFPVNYVGECIRTVPYTDPDHASLKILARLMTAKFLHTEIREKGGAYGGGAKLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pitrilysin metallopeptidase 1
别名
计算分子量 60 kDa, 117 kDa
观测分子量110-120 kDa
GenBank蛋白编号BC001150
基因名称 PITRM1
Gene ID (NCBI) 10531
RRIDAB_3086385
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5JRX3
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PITRM1 (Presequence protease, mitochondrial) is a ATP-independent protease that degrades mitochondrial transit peptides after their cleavage.It is specific for peptides in the range of 10 to 65 residues.and able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease.It shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference(PMID:16849325).This protein has a calculated molecular mass of 117kD and it is expressed widely.

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