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验证数据展示

  • IHC staining of human placenta using 21169-1-AP

    IHC staining of human placenta using 21169-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21169-1-AP (PITPNM2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse placenta using 21169-1-AP

    IHC staining of mouse placenta using 21169-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 21169-1-AP (PITPNM2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PITPNM2/NIR3 Polyclonal antibody

PITPNM2/NIR3 Polyclonal Antibody for IHC, ELISA
Cat No. 21169-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA

别名

PITPNM2, KIAA1457, Membrane-associated phosphatidylinositol transfer protein 2, NIR3, NIR-3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman placenta tissue, mouse placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21169-1-AP targets PITPNM2/NIR3 in IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag15385

Product name: Recombinant human PITPNM2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 233-328 aa of BC141813

Sequence: EWYGLSMENIRELEKEAQLMLSRKMAQFNEDGEEATELVKHEAVSDQTSGEPPEPSSSNGEPLVGRGLKKQWSTSSKSSRSSKRGASPSRHSISEW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphatidylinositol transfer protein, membrane-associated 2
别名 PITPNM2, KIAA1457, Membrane-associated phosphatidylinositol transfer protein 2, NIR3, NIR-3
计算分子量 1349 aa, 149 kDa
GenBank蛋白编号BC141813
基因名称 PITPNM2
Gene ID (NCBI) 57605
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BZ72
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PITPNM2, also known as NIR3, belongs to a family of membrane-associated phosphatidylinositol transfer domain-containing proteins that share homology with the Drosophila retinal degeneration B (rdgB) protein(PMID: 15627748). PITPNM2/NIR3 promotes both TCRβ selection and positive selection in thymocytes. PITPNM2/NIR3 is required for PIP2 replenishment and TCR-induced calcium signaling in response to weak TCR stimulation(PMID: 36581712).

实验方案

Product Specific Protocols
IHC protocol for PITPNM2/NIR3 antibody 21169-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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