验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
| IF | See 1 publications below |
产品信息
20990-1-AP targets PIRT in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | IF, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | phosphoinositide-interacting regulator of transient receptor potential channels |
| 别名 | hCG_1776018, PIRT, TRPV1 complex |
| 计算分子量 | 15 kDa |
| 观测分子量 | 25 kDa |
| GenBank蛋白编号 | NM_001101387 |
| 基因名称 | PIRT |
| Gene ID (NCBI) | 644139 |
| RRID | AB_2878785 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P0C851 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PIRT is a key component of the TRPV1 complex that positively regulates TRPV1 activity. It binds various phosphoinositide, including phosphatidylinositol 4,5-bisphosphate (PIP2), but not phosphatidylinositol (PI). PIRT is a membrane protein with MW 15-25 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PIRT antibody 20990-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO Rep Defective branched-chain amino acid catabolism in dorsal root ganglia contributes to mechanical pain | ||
Evid Based Complement Alternat Med C-Phycocyanin Alleviates Bladder Inflammation and Dysfunction in Cyclophosphamide-Induced Cystitis in a Mouse Model by Inhibiting COX-2 and EP4. |